ArticleAnalytical and bioanalytical chemistry2020
Fluorescence calibration standards made from broadband emitters encapsulated in polymer beads for fluorescence microscopy and flow cytometry.
Article in Analytical and bioanalytical chemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Validated workflows for preparing and characterizing core-stained and surface-labeled fluorescent polymer particles with simple commercial automation tools.Analytical and bioanalytical chemistry · 2026Article
- Simple, cost-effective, highly stable solid-state photoluminescence standard for fluorometer calibration in low-resource settings.Optics continuum · 2024Article
- Correlating semiconductor nanoparticle architecture and applicability for the controlled encoding of luminescent polymer microparticles.Scientific reports · 2024Article
- Exploring Simple Particle-Based Signal Amplification Strategies in a Heterogeneous Sandwich Immunoassay with Optical Detection.Analytical chemistry · 2024Article
- Efficiency scale for scattering luminescent particles linked to fundamental and measurable spectroscopic properties.Scientific reports · 2023Article
- Lifetime encoding in flow cytometry for bead-based sensing of biomolecular interaction.Scientific reports · 2020Article
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Authors and funding
3 authors.
Funding
Abstract
We present here the design and characterization of a set of spectral calibration beads. These calibration beads are intended for the determination and regular control of the spectral characteristics of fluorescence microscopes and other fluorescence measuring devices for the readout of bead-based assays. This set consists of micrometer-sized polymer beads loaded with dyes from the liquid Calibration Kit Spectral Fluorescence Standards developed and certified by BAM for the wavelength-dependent determination of the spectral responsivity of fluorescence measuring devices like spectrofluorometers. To cover the wavelength region from 400 to 800 nm, two new near-infrared emissive dyes were included, which were spectroscopically characterized in solution and encapsulated in the beads. The resulting set of beads presents the first step towards a new platform of spectral calibration beads for the determination of the spectral characteristics of fluorescence instruments like fluorescence microscopes, FCM setups, and microtiter plate readers, thereby meeting the increasing demand for reliable and comparable fluorescence data especially in strongly regulated areas, e.g., medical diagnostics. This will eventually provide the basis for standardized calibration procedures for imaging systems as an alternative to microchannel slides containing dye solutions previously reported by us. Graphical abstract.
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