ArticleCommunications biology2020
Real-time monitoring of live mycobacteria with a microfluidic acoustic-Raman platform.
Article in Communications biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 45 citations in OpenAlex.
- Combined Approach of Chromatographic Fractionation and Raman Spectroscopy for Metabolite Profiling ofInternational journal of molecular sciences · 2026Article
- Ultrasmall episymbiont Nanosynbacter lyticus employs multiple ATP-generating metabolic pathways during horizontal transmission.The ISME journal · 2026Article
- Future Research Directions on Mycobacterium tuberculosis Proteins.Advances in experimental medicine and biology · 2026Review
- Microfluidics to Meet Antibiotic Resistance: A Growing Research Frontier.Antibiotics (Basel, Switzerland) · 2025Review
- Massively parallel microbubble nano-assembly.Nature communications · 2025Article
- Phenotypic drug susceptibility testing for Mycobacterium tuberculosis variant bovis BCG in 12 hours.Nature communications · 2025Article
- Development of a Mass-Producible Microfluidic Device for Single and Bulk Mycobacteria Investigations.Biosensors · 2025Article
- Recent Advances in Dielectrophoretic Manipulation and Separation of Microparticles and Biological Cells.Biosensors · 2024Review
- Raman-Activated, Interactive Sorting of Isotope-Labeled Bacteria.Sensors (Basel, Switzerland) · 2024Article
- Constrained Volume Micro- and Nanoparticle Collection Methods in Microfluidic Systems.Micromachines · 2024Review
- Episymbiotic Saccharibacteria induce intracellular lipid droplet production in their host bacteria.The ISME journal · 2024Article
- Rapid Drug Susceptibility Testing to Preserve Antibiotics.Methods in molecular biology (Clifton, N.J.) · 2024Review
- Monitoring Live Mycobacteria in Real-Time Using a Microfluidic Acoustic-Raman Platform.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Accessing Properties of Molecular Compounds Involved in Cellular Metabolic Processes with Electron Paramagnetic Resonance, Raman Spectroscopy, and Differential Scanning Calorimetry.Molecules (Basel, Switzerland) · 2023Review
- Nanogap traps for passive bacteria concentration and single-point confocal Raman spectroscopy.Biomicrofluidics · 2023Article
- Implications of drug-induced phenotypical resistance: Is isoniazid radicalizingFrontiers in antibiotics · 2022Article
- Evolution of Antibacterial Drug Screening Methods: Current Prospects for Mycobacteria.Microorganisms · 2021Review
- Raman Imaging and Fluorescence Lifetime Imaging Microscopy for Diagnosis of Cancer State and Metabolic Monitoring.Cancers · 2021Review
- Microfluidics as a Novel Technique for Tuberculosis: From Diagnostics to Drug Discovery.Microorganisms · 2021Review
- Revealing Antibiotic Tolerance of theAntibiotics (Basel, Switzerland) · 2021Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tuberculosis (TB) remains a leading cause of death worldwide. Lipid rich, phenotypically antibiotic tolerant, bacteria are more resistant to antibiotics and may be responsible for relapse and the need for long-term TB treatment. We present a microfluidic system that acoustically traps live mycobacteria, M. smegmatis, a model organism for M. tuberculosis. We then perform optical analysis in the form of wavelength modulated Raman spectroscopy (WMRS) on the trapped M. smegmatis for up to eight hours, and also in the presence of isoniazid (INH). The Raman fingerprints of M. smegmatis exposed to INH change substantially in comparison to the unstressed condition. Our work provides a real-time assessment of the impact of INH on the increase of lipids in these mycobacteria, which could render the cells more tolerant to antibiotics. This microfluidic platform may be used to study any microorganism and to dynamically monitor its response to different conditions and stimuli.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.