ArticleScientific reports2020
Giant magnetoresistive biosensors for real-time quantitative detection of protease activity.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 44 citations in OpenAlex.
- Detection of factor Xa activity using giant magnetoresistive biosensors.Analytica chimica acta · 2024Article
- Surface-modified CMOS biosensors.Frontiers in bioengineering and biotechnology · 2024Review
- A GMR enzymatic assay for quantifying nuclease and peptidase activity.Frontiers in bioengineering and biotechnology · 2024Article
- Structural Transitions of Papain-like Cysteine Proteases: Implications for Sensor Development.Biomimetics (Basel, Switzerland) · 2023Article
- Proteolytic Biosensors with Functional Nanomaterials: Current Approaches and Future Challenges.Biosensors · 2023Review
- A GMR-based assay for quantification of the human response to influenza.Biosensors & bioelectronics · 2022Article
- Giant Magnetoresistance Biosensors in Biomedical Applications.ACS applied materials & interfaces · 2022Review
- Study on Multi-Model Soft Sensor Modeling Method and Its Model Optimization for the Fermentation Process ofSensors (Basel, Switzerland) · 2021Article
- Aspects of high-performance and bio-acceptable magnetic nanoparticles for biomedical application.Asian journal of pharmaceutical sciences · 2021Review
- Two-dimensional arrays of vertically packed spin-valves with picoTesla sensitivity at room temperature.Scientific reports · 2021Article
- Detection of pathogenic bacteria by magneto-immunoassays: a review.Journal of biomedical research · 2020Article
- A Paper-Based Near-Infrared Optical Biosensor for Quantitative Detection of Protease Activity Using Peptide-Encapsulated SWCNTs.Sensors (Basel, Switzerland) · 2020Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Proteases are enzymes that cleave proteins and are crucial to physiological processes such as digestion, blood clotting, and wound healing. Unregulated protease activity is a biomarker of several human diseases. Synthetic peptides that are selectively hydrolyzed by a protease of interest can be used as reporter substrates of unregulated protease activity. We developed an activity-based protease sensor by immobilizing magnetic nanoparticles (MNPs) to the surface of a giant magnetoresistive spin-valve (GMR SV) sensor using peptides. Cleavage of these peptides by a protease releases the magnetic nanoparticles resulting in a time-dependent change in the local magnetic field. Using this approach, we detected a significant release of MNPs after 3.5 minutes incubation using just 4 nM of the cysteine protease, papain. In addition, we show that proteases in healthy human urine do not release the MNPs, however addition of 20 nM of papain to the urine samples resulted in a time-dependent change in magnetoresistance. This study lays the foundation for using GMR SV sensors as a platform for real-time, quantitative detection of protease activity in biological fluids.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.