ArticleMicroorganisms2020
Cloacal Swabs Are Unreliable Sources for Estimating Lower Gastro-Intestinal Tract Microbiota Membership and Structure in Broiler Chickens.
Article in Microorganisms, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed.
- Gut check: Exploring tools, techniques, and future directions in microbiome research.Poultry science · 2026Article
- The elephant (bird) in the room: unknown mechanisms and unresolved impacts of low DNA yields in avian microbiome research.Animal microbiome · 2026Article
- Molecular detection of CTX-M ESBL-encoding genes inOpen veterinary journal · 2026Article
- Diverse novel and avian-associated viruses in the ileal viromes of Northern Mockingbird (Mimus polyglottos).Archives of virology · 2026Article
- Conjugation frequency of ESBL- and pAmpC- E. coli in broiler chickens in vivo and in vitro.BMC microbiology · 2026Article
- Cotton farming affects ileal virome in a sedentary wild passerine.Animal microbiome · 2026Article
- Metagenomic Profiling of Fecal and Cecal Microbiota and Their Antimicrobial Resistance Genes in Indigenous Backyard Poultry.International journal of microbiology · 2026Article
- Molecular characterization of Salmonella isolates from poultry in Egypt: Detection of virulence, ESBLs genes and antimicrobial resistance profiling.Veterinary research communications · 2025Article
- Do we need a standardized 16S rRNA gene amplicon sequencing analysis protocol for poultry microbiota research?Poultry science · 2025Review
- Bacterial communities along parrot digestive and respiratory tracts: the effects of sample type, species and time.International microbiology : the official journal of the Spanish Society for Microbiology · 2024Article
- Comparative study of the gut microbial communities collected by scraping and swabbing in a fish model: a comprehensive guide to promote non-lethal procedures for gut microbial studies.Frontiers in veterinary science · 2024Article
- Effect of dietary fat source on the composition of the cecal microbiome in maturing broiler chicken.Frontiers in microbiology · 2024Article
- Article
- Phylogenetically under-dispersed gut microbiomes are not correlated with host genomic heterozygosity in a genetically diverse reptile community.Molecular ecology · 2023Article
- 16S rRNA gene-based assessment of common broiler chicken sampling methods: Evaluating intra-flock sample size, cecal pair similarity, and cloacal swab similarity to other alimentary tract locations.Frontiers in physiology · 2022Article
- Impact of Dietary Egg Yolk IgY Powder on Behavior, Meat Quality, Physiology, and IntestinalFrontiers in veterinary science · 2022Article
- Phytogenic Ingredients from Hops and Organic Acids Improve Selected Indices of Welfare, Health Status Markers, and Bacteria Composition in the Caeca of Broiler Chickens.Animals : an open access journal from MDPI · 2021Article
- Choice of Commercial DNA Extraction Method Does Not Affect 16S Sequencing Outcomes in Cloacal Swabs.Animals : an open access journal from MDPI · 2021Article
- Temporal Dynamics of Cloacal Microbiota in Adult Laying Chickens With and Without Access to an Outdoor Range.Frontiers in microbiology · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The gastrointestinal microbiota of chickens plays a central role in health and performance. Cloacal swabs, due to their proximity to the ceca (a vital site of functional activity), are an alternative, non-invasive method used for assaying microbial communities and might be a viable option for longitudinal studies. In this study, the microbiota of twenty paired cecal content and cloacal swab samples representing two dietary treatments was assessed using 16S rRNA V4 hypervariable region sequencing and was analyzed using the MOTHUR pipeline, Phyloseq, and Vegan packages. Paired t-test and Wilcoxon signed-rank tests showed significant differences in the Chao1 index (
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.