ReviewJournal of biomedical optics2020
Fluorescence lifetime imaging microscopy: fundamentals and advances in instrumentation, analysis, and applications.
Review in Journal of biomedical optics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 398 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
398 citing papers in PubMed, 1 synthesis or guideline pooled it, 812 citations in OpenAlex.
- Predicting immune checkpoint inhibitors response via fluorescence lifetime imaging microscopy: a systematic review.Frontiers in immunology · 2025Pooled it
- Superconducting nanowire single-photon detectors for enhanced biomedical imaging.Journal of biomedical optics · 2026Article
- Development of solid-state fluorescence lifetime standards for clinical applications using dyed epoxy resins.Journal of biomedical optics · 2026Article
- Deep learning-enhanced single-shot triorganelle STED-FLIM imaging of lipid dynamics in living cells.Science advances · 2026Article
- Neural Network-Based System for Rapid Diagnostic Decision Support Using FLIM Data: Segmentation and Classification in Liver, Skin, and Pancreatic Tissues.International journal of molecular sciences · 2026Article
- Early Postmortem Changes in NADH Fluorescence Lifetime Parameters in Rat Skeletal Muscle: Potential for Postmortem Interval Estimation.International journal of molecular sciences · 2026Article
- Excitonic and Optical Transduction Mechanisms in Quantum Dot Sensors for Environmental Pollutant Detection.Sensors (Basel, Switzerland) · 2026Review
- FLIMExplorer: interactive GUI for object-based visualization and analysis of fluorescence lifetime images.JPhys photonics · 2026Article
- The multifaceted significance of phosphoinositides in endocytic trafficking.FEBS letters · 2026Review
- Super-resolution imaging using fluorescence-lifetime emission-difference.Biomedical optics express · 2026Article
- Genetically encoded tools for tracking metabolites in live cells.Biochemical Society transactions · 2026Review
- A single-chip, dual-gain color-NIR camera for fluorescence-guided lung and breast cancer surgery.Light, science & applications · 2026Article
- Targeted Near-Infrared Photoacoustic Probes for Dual-Channel Cartilage and Bone Imaging.Angewandte Chemie (International ed. in English) · 2026Article
- FLIM Playground: An interactive, end-to-end graphical user interface for analyzing single cells with fluorescence lifetime imaging microscopy.Cell reports methods · 2026Article
- Large-FOV, dual-region, two-photon fluorescence lifetime imaging microscopy (LD-2P-FLIM) for multiparameter and quantitative brain function imaging.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Multimodal Raman and Fluorescence Lifetime Imaging: A New Analytical Approach for Resolving Timing-Dependent Metabolic Recovery in Endothelial Dysfunction.Analytical chemistry · 2026Article
- Zero-Potential No-Discharge APCI Combined With pH and Colorimetric Measurements Reveals a Protein-Dependent Change in Albumin Proton Affinity Under Quantum-Dot Patch Exposure.Journal of mass spectrometry : JMS · 2026Article
- Advanced imaging of membrane contact sites.Nature cell biology · 2026Review
- Development of far-red fluorescent proteins for temporal domain multiplexing and super-resolution imaging.Cell reports methods · 2026Article
- Luminescence lifetime imaging integrated into selective plane illumination microscopy for oxygen imaging in 3D cell cultures.Biomedical optics express · 2026Article
338 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
significanceFluorescence lifetime imaging microscopy (FLIM) is a powerful technique to distinguish the unique molecular environment of fluorophores. FLIM measures the time a fluorophore remains in an excited state before emitting a photon, and detects molecular variations of fluorophores that are not apparent with spectral techniques alone. FLIM is sensitive to multiple biomedical processes including disease progression and drug efficacy.
aimWe provide an overview of FLIM principles, instrumentation, and analysis while highlighting the latest developments and biological applications. APPROACH: This review covers FLIM principles and theory, including advantages over intensity-based fluorescence measurements. Fundamentals of FLIM instrumentation in time- and frequency-domains are summarized, along with recent developments. Image segmentation and analysis strategies that quantify spatial and molecular features of cellular heterogeneity are reviewed. Finally, representative applications are provided including high-resolution FLIM of cell- and organelle-level molecular changes, use of exogenous and endogenous fluorophores, and imaging protein-protein interactions with Förster resonance energy transfer (FRET). Advantages and limitations of FLIM are also discussed.
conclusionsFLIM is advantageous for probing molecular environments of fluorophores to inform on fluorophore behavior that cannot be elucidated with intensity measurements alone. Development of FLIM technologies, analysis, and applications will further advance biological research and clinical assessments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.