ArticleJournal of clinical virology : the official publication of the Pan American Society for Clinical Virology2020
Validation of SARS-CoV-2 detection across multiple specimen types.
Article in Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers, 4 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
50 citing papers in PubMed, 4 syntheses or guidelines pooled it, 75 citations in OpenAlex.
- SARS-CoV-2 saliva testing using RT-PCR: a systematic review.International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases · 2022Pooled it
- Cerebrospinal fluid from COVID-19 patients with olfactory/gustatory dysfunction: A review.Clinical neurology and neurosurgery · 2021Pooled it
- Cerebrospinal fluid in COVID-19: A systematic review of the literature.Journal of the neurological sciences · 2021Pooled it
- Systematic review with meta-analysis: SARS-CoV-2 stool testing and the potential for faecal-oral transmission.Alimentary pharmacology & therapeutics · 2020Pooled it
- Testing for SARS-CoV-2: lessons learned and current use cases.Clinical microbiology reviews · 2024Review
- Internationally standardized respiratory viral load testing with limited resources: A derivative-of-care calibration strategy for SARS-CoV-2.Influenza and other respiratory viruses · 2024Article
- Automatic diagnosis of COVID-19 from CT images using CycleGAN and transfer learning.Applied soft computing · 2023Article
- Commercially available SARS-CoV-2 RT-qPCR diagnostic tests need obligatory internal validation.Scientific reports · 2023Article
- Agnostic Sequencing for Detection of Viral Pathogens.Clinical microbiology reviews · 2023Review
- Evaluation and clinical validation of monkeypox (mpox) virus real-time PCR assays.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2023Article
- Singleplex, multiplex and pooled sample real-time RT-PCR assays for detection of SARS-CoV-2 in an occupational medicine setting.Scientific reports · 2022Article
- Second round of an interlaboratory comparison of SARS-CoV2 molecular detection assays used by 45 veterinary diagnostic laboratories in the United States.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2022Article
- The Successes and Challenges of SARS-CoV-2 Molecular Testing in the United States.Clinics in laboratory medicine · 2022Review
- Cycle Threshold Values from Severe Acute Respiratory Syndrome Coronavirus-2 Reverse Transcription-Polymerase Chain Reaction Assays: Interpretation and Potential Use Cases.Clinics in laboratory medicine · 2022Review
- Co-infection of human immunodeficiency virus, herpes simplex virus-2 and SARS-CoV-2 in a patient with false-negative real-time polymerase chain reaction results.Singapore medical journal · 2022Article
- Host-pathogen dynamics in longitudinal clinical specimens from patients with COVID-19.Scientific reports · 2022Article
- SARS-CoV-2 Viral RNA Is Detected in the Bone Marrow in Post-Mortem Samples Using RT-LAMP.Diagnostics (Basel, Switzerland) · 2022Article
- Mutations in viral nucleocapsid protein and endoRNase are discovered to associate with COVID19 hospitalization risk.Scientific reports · 2022Article
- Article
- Molecular docking of SARS-COV-2 Spike epitope sequences identifies heterodimeric peptide-protein complex formation with human Zo-1, TLR8 and brain specific glial proteins.Medical hypotheses · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSevere Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has caused considerable disruption across the world, resulting in more than 235,000 deaths since December 2019. SARS-CoV-2 has a wide tropism and detection of the virus has been described in multiple specimen types, including various respiratory secretions, cerebrospinal fluid, and stool.
objectiveTo evaluate the accuracy and sensitivity of a laboratory modified CDCbased SARS-CoV-2 N1 and N2 assay across a range of sample types. Study Design We compared the matrix effect on the analytical sensitivity of SARS-CoV-2 detection by qRT-PCR in nasal swabs collected in viral transport medium (VTM), bronchoalveolar lavage (BAL), sputum, plasma, cerebral spinal fluid (CSF), stool, VTM, phosphate buffered saline (PBS), and Hanks' Balanced Salt Solution (HBSS). Initial limits of detection (LoD) were subsequently narrowed to confirm an LoD for each specimen type and target gene.
resultsLoDs were established using a modified CDC-based laboratory developed test and ranged from a mean CT cut-off of 33.8-35.7 (10-20 copies/reaction) for the N1 gene target, and 34.0-36.2 (1-10 copies/reaction) for N2. Alternatives to VTM such as PBS and HBSS had comparable LoDs. The N2 gene target was found to be most sensitive in CSF.
conclusionA modified CDC-based laboratory developed test is able to detect SARSCoV- 2 accurately with similar sensitivity across all sample types tested.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.