ArticleProceedings of the National Academy of Sciences of the United States of America2020
Single-nucleus RNA sequencing of mouse auditory cortex reveals critical period triggers and brakes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 38 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
38 citing papers in PubMed, 1 synthesis or guideline pooled it, 65 citations in OpenAlex.
- Time Window of the Critical Period for Neuroplasticity in S1, V1, and A1 Sensory Areas of Small Rodents: A Systematic Review.Frontiers in neuroanatomy · 2022Pooled it
- Soluble Nogo-Receptor-Fc decoy (AXER-204) in patients with chronic cervical spinal cord injury in the USA: a first-in-human and randomised clinical trial.The Lancet. Neurology · 2023Trial
- Developing mouse inhibitory neuron single-cell transcriptomes reveal distinct modes of cell-type diversification.Nature neuroscience · 2026Article
- Molecular taxonomy and spatial organization define neuronal subtypes in the mouse inferior colliculus.iScience · 2026Article
- Age-Dependent Auditory Plasticity: Critical Periods in Subcortical and Cortical Encoding.Journal of audiology & otology · 2026Review
- Simultaneous Recordings of Neuronal Activities, Pup Calls, and Maternal Behaviors in Lactating Mice.eNeuro · 2026Article
- Investigating hierarchical critical periods in human neurodevelopment.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2026Review
- The Development of Neural Inhibition across Species: Insights from the Hurst Exponent.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- A single-cell transcriptomic atlas of developing inhibitory neurons reveals expanding and contracting modes of diversification.bioRxiv : the preprint server for biology · 2025Article
- Leveraging ultra-high field (7T) MRI in psychiatric research.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2024Review
- Opportunities and Challenges in Advancing Plant Research with Single-cell Omics.Genomics, proteomics & bioinformatics · 2024Review
- Gene-environmental regulation of the postnatal post-mitotic neuronal maturation.Trends in genetics : TIG · 2024Review
- Brain-derived neurotrophic factor from microglia regulates neuronal development in the medial prefrontal cortex and its associated social behavior.Molecular psychiatry · 2024Article
- STAB2: an updated spatio-temporal cell atlas of the human and mouse brain.Nucleic acids research · 2024Article
- Molecular and functional profiling of cell diversity and identity in the lateral superior olive, an auditory brainstem center with ascending and descending projections.Frontiers in cellular neuroscience · 2024Article
- Learning induces unique transcriptional landscapes in the auditory cortex.Hearing research · 2023Review
- A critical period plasticity framework for the sensorimotor-association axis of cortical neurodevelopment.Trends in neurosciences · 2023Review
- Application of single-cell multi-omics approaches in horticulture research.Molecular horticulture · 2023Review
- Learning induces unique transcriptional landscapes in the auditory cortex.bioRxiv : the preprint server for biology · 2023Article
- Transcriptional Profile Changes after Noise-Induced Tinnitus in Rats.Brain sciences · 2023Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Auditory experience drives neural circuit refinement during windows of heightened brain plasticity, but little is known about the genetic regulation of this developmental process. The primary auditory cortex (A1) of mice exhibits a critical period for thalamocortical connectivity between postnatal days P12 and P15, during which tone exposure alters the tonotopic topography of A1. We hypothesized that a coordinated, multicellular transcriptional program governs this window for patterning of the auditory cortex. To generate a robust multicellular map of gene expression, we performed droplet-based, single-nucleus RNA sequencing (snRNA-seq) of A1 across three developmental time points (P10, P15, and P20) spanning the tonotopic critical period. We also tone-reared mice (7 kHz pips) during the 3-d critical period and collected A1 at P15 and P20. We identified and profiled both neuronal (glutamatergic and GABAergic) and nonneuronal (oligodendrocytes, microglia, astrocytes, and endothelial) cell types. By comparing normal- and tone-reared mice, we found hundreds of genes across cell types showing altered expression as a result of sensory manipulation during the critical period. Functional voltage-sensitive dye imaging confirmed GABA circuit function determines critical period onset, while Nogo receptor signaling is required for its closure. We further uncovered previously unknown effects of developmental tone exposure on trajectories of gene expression in interneurons, as well as candidate genes that might execute tonotopic plasticity. Our single-nucleus transcriptomic resource of developing auditory cortex is thus a powerful discovery platform with which to identify mediators of tonotopic plasticity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.