ArticleJournal of clinical virology : the official publication of the Pan American Society for Clinical Virology2020
A RT-PCR assay for the detection of coronaviruses from four genera.
Article in Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
What it found
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Who cites it
47 citing papers in PubMed, 65 citations in OpenAlex.
- First Detection of Porcine Hemagglutinating Encephalomyelitis Virus (PHEV) in Wild Boars (Veterinary sciences · 2026Article
- Detection and complete genome characterisation of bat coronaviruses from Ghana.Archives of virology · 2026Article
- Surveillance of Zoonotic Pathogens and Taxonomic Identification of Non-volant Small Mammals in Peninsular Malaysia.Tropical life sciences research · 2026Article
- Surveillance of Swine Coronaviruses in Hungarian Herds with a Newly Established Pan-Coronavirus RT-PCR System.Animals : an open access journal from MDPI · 2026Article
- Detection and Genomic Characterization of Novel Respiratory Viruses in US and Mexican Cattle Farms.Transboundary and emerging diseases · 2026Article
- The coronaviral landscape across diverse mammalian species in the Northeastern United States.Scientific reports · 2025Article
- Proteomic Signatures of SARS-CoV-2 Susceptibility in Mexican Free-tailed Bats and Their Application to Viral Surveillance.Integrative and comparative biology · 2025Article
- Diverse respiratory viruses detected among hospitalized patients with pneumonia in Sri Lanka and Vietnam.IJID regions · 2025Article
- Investigation of coronaviruses, paramyxoviruses, and poxviruses in free-ranging anteaters and armadillos: a search for the unknown in Brazil.Archives of virology · 2025Article
- Article
- Integrating One Health research in Sarawak, Malaysia: Addressing emerging andOne health (Amsterdam, Netherlands) · 2025Review
- Monitoring for respiratory viruses among wild canids, Texas.One health (Amsterdam, Netherlands) · 2025Article
- Comparative Analysis of Quan and Watanabe Pan-Coronavirus Assays for Bat Coronavirus Diversity in Sarawak, East Malaysia.Journal of medical virology · 2025Article
- Article
- Detection of an Alphacoronavirus in a Brazilian Bat (Molossus sp.).Journal of molecular evolution · 2025Article
- Novel Rodent Coronavirus-like Virus Detected Among Beef Cattle with Respiratory Disease in Mexico.Viruses · 2025Article
- Avian Migration-Mediated Transmission and Recombination Driving the Diversity of Gammacoronaviruses and Deltacoronaviruses.Molecular biology and evolution · 2025Article
- Investigation of the genome, taxonomy, and distribution of pigeon gammacoronavirus: insights into its relationships with other avian gammacoronaviruses.Frontiers in microbiology · 2025Article
- No Evidence of Novel Respiratory Viruses on Two Texas Dairy Farms Before the H5N1 Avian Influenza Virus Epizootic.Influenza and other respiratory viruses · 2025Article
- Article
Corrections and comments
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Authors and funding
11 authors at 5 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDuring the past two decades, three novel coronaviruses (CoVs) have emerged to cause international human epidemics with severe morbidity. CoVs have also emerged to cause severe epidemics in animals. A better understanding of the natural hosts and genetic diversity of CoVs are needed to help mitigate these threats.
objectiveTo design and evaluate a molecular diagnostic tool for detection and identification of all currently recognized and potentially future emergent CoVs from the Orthocoronavirinae subfamily. STUDY DESIGN AND
resultsWe designed a semi-nested, reverse transcription RT-PCR assay based upon 38 published genome sequences of human and animal CoVs. We evaluated this assay with 14 human and animal CoVs and 11 other non-CoV respiratory viruses. Through sequencing the assay's target amplicon, the assay correctly identified each of the CoVs; no cross-reactivity with 11 common respiratory viruses was observed. The limits of detection ranged from 4 to 4 × 10
conclusionsThis assay can be used for detection and identification of all previously recognized CoVs, including SARS-CoV-2, and potentially any emergent CoVs in the Orthocoronavirinae subfamily.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.