ReviewTrends in biochemical sciences2020
Re-Evaluating the Conventional Wisdom about Binding Assays.
Review in Trends in biochemical sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed.
- Advanced biosensing strategies for high-risk foodborne pathogens: a comprehensive review ofFood chemistry. Molecular sciences · 2026Review
- Single-molecule readout of reversible nanoswitches enables continuous monitoring of low biomarker concentrations.Nature communications · 2026Article
- Multidomain Molecular Sensor Devices, Systems, and Algorithms for Improved Physiological Monitoring.Micromachines · 2025Review
- Synthetic CRISPR Networks Driven by Transcription Factors via Structure-Switching DNA Translators.Journal of the American Chemical Society · 2025Article
- Article
- High-affinity one-step aptamer selection using a non-fouling porous hydrogel.Nature biotechnology · 2024Article
- Fluorometric and Colorimetric Method for SARS-CoV-2 Detection Using Designed Aptamer Display Particles.Biosensors · 2024Article
- Structural and biochemical characterization establishes a detailed understanding of KEAP1-CUL3 complex assembly.Free radical biology & medicine · 2023Article
- Scalable biomimetic sensing system with membrane receptor dual-monolayer probe and graphene transistor arrays.Science advances · 2023Article
- Extending the dynamic range of biomarker quantification through molecular equalization.Nature communications · 2023Article
- Article
- A high-dimensional microfluidic approach for selection of aptamers with programmable binding affinities.Nature chemistry · 2023Article
- Biomolecular sensors for advanced physiological monitoring.Nature reviews bioengineering · 2023Review
- Conformational-switch biosensors as novel tools to support continuous, real-time molecular monitoring in lab-on-a-chip devices.Lab on a chip · 2023Review
- Pre-equilibrium biosensors as an approach towards rapid and continuous molecular measurements.Nature communications · 2022Article
- Dielectrophoresis: An Approach to Increase Sensitivity, Reduce Response Time and to Suppress Nonspecific Binding in Biosensors?Biosensors · 2022Review
- A direct high-throughput protein quantification strategy facilitates discovery and characterization of a celastrol-derived BRD4 degrader.Cell chemical biology · 2022Article
- A Folding-Based Electrochemical Aptasensor for the Single-Step Detection of the SARS-CoV-2 Spike Protein.ACS applied materials & interfaces · 2022Article
- Detection ofSensors (Basel, Switzerland) · 2022Article
- Sensing Methodology for the Rapid Monitoring of Biomolecules at Low Concentrations over Long Time Spans.ACS sensors · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
Analytical technologies based on binding assays have evolved substantially since their inception nearly 60 years ago, but our conceptual understanding of molecular recognition has not kept pace. Contemporary technologies, such as single-molecule and digital measurements, have challenged, or even rendered obsolete, core concepts behind conventional binding assay design. Here, we explore the fundamental principles underlying molecular recognition systems, which we consider in terms of signals generated through concentration-dependent shifts in equilibrium. We challenge certain orthodoxies related to binding-based detection assays, including the primary importance of a low dissociation constant (K
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.