ArticleCell reports2020
Iterative Single-Cell Analyses Define the Transcriptome of the First Functional Hematopoietic Stem Cells.
Article in Cell reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
What it found
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Who cites it
44 citing papers in PubMed, 68 citations in OpenAlex.
- Fetal-restricted hematopoietic progenitors arise from hemogenic endothelium in vitelline and umbilical arteries.Nature cardiovascular research · 2026Article
- Mechanometabolism instructs hematopoietic stem cell specification.The Journal of experimental medicine · 2026Article
- Apelin signaling acts as a molecular switch between endothelial and hematopoietic stem cell fates.EMBO reports · 2026Article
- CD43, but not CD41 or GPI-80, marks first definitive haematopoietic stem cells in the human embryo.Development (Cambridge, England) · 2025Article
- Unraveling hematopoietic stem cell ontogeny through single-cell multi-omics approaches.Frontiers in cell and developmental biology · 2025Review
- NR4A1 and NR4A2 orphan nuclear receptors regulate endothelial-to-hematopoietic transition in mouse hematopoietic stem cell specification.Development (Cambridge, England) · 2024Article
- New insights into the endothelial origin of hematopoietic system inspired by "TIF" approaches.Blood science (Baltimore, Md.) · 2024Review
- Mitochondrial permeability transition dictates mitochondrial maturation upon switch in cellular identity of hematopoietic precursors.Communications biology · 2024Article
- Transition of signal requirement in hematopoietic stem cell development from hemogenic endothelial cells.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Systematic single-cell analysis reveals dynamic control of transposable element activity orchestrating the endothelial-to-hematopoietic transition.BMC biology · 2024Article
- Runx1+ vascular smooth muscle cells are essential for hematopoietic stem and progenitor cell development in vivo.Nature communications · 2024Article
- Cis inhibition of NOTCH1 through JAGGED1 sustains embryonic hematopoietic stem cell fate.Nature communications · 2024Article
- Deciphering hematopoietic stem cell development: key signaling pathways and mechanisms.Frontiers in cell and developmental biology · 2024Review
- Haematopoietic development and HSC formation in vitro: promise and limitations of gastruloid models.Emerging topics in life sciences · 2023Review
- Haematopoietic stem and progenitor cell heterogeneity is inherited from the embryonic endothelium.Nature cell biology · 2023Article
- Gene expression networks involved in multiple cellular programs coexist in individual hepatocellular cancer cells.Heliyon · 2023Article
- Gata2-regulated Gfi1b expression controls endothelial programming during endothelial-to-hematopoietic transition.Blood advances · 2023Article
- Hematopoietic Stem Cell Development in Mammalian Embryos.Advances in experimental medicine and biology · 2023Review
- Unveiling the complexity of transcription factor networks in hematopoietic stem cells: implications for cell therapy and hematological malignancies.Frontiers in oncology · 2023Review
- GATA2 deficiency and MDS/AML: Experimental strategies for disease modelling and future therapeutic prospects.British journal of haematology · 2022Review
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
Abstract
Whereas hundreds of cells in the mouse embryonic aorta transdifferentiate to hematopoietic cells, only very few establish hematopoietic stem cell (HSC) identity at a single time point. The Gata2 transcription factor is essential for HSC generation and function. In contrast to surface-marker-based cell isolation, Gata2-based enrichment provides a direct link to the internal HSC regulatory network. Here, we use iterations of index-sorting of Gata2-expressing intra-aortic hematopoietic cluster (IAHC) cells, single-cell transcriptomics, and functional analyses to connect HSC identity to specific gene expression. Gata2-expressing IAHC cells separate into 5 major transcriptomic clusters. Iterative analyses reveal refined CD31, cKit, and CD27 phenotypic parameters that associate specific molecular profiles in one cluster with distinct HSC and multipotent progenitor function. Thus, by iterations of single-cell approaches, we identify the transcriptome of the first functional HSCs as they emerge in the mouse embryo and localize them to aortic clusters containing 1-2 cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.