ArticlePloS one2020
Rediscovering an old foe: Optimised molecular methods for DNA extraction and sequencing applications for fungarium specimens of powdery mildew (Erysiphales).
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Whole genome sequencing of historical specimens from the world's largest fungal collection yields high-quality assemblies.The New phytologist · 2026Article
- Utilizing multi-trait selection indices for genetic characterization and SSR marker validation linked to powdery mildew resistance in sunflower.Scientific reports · 2026Article
- What are the 100 most cited fungal genera?Studies in mycology · 2024Article
- Deciphering the Genomic Landscape and Virulence Mechanisms of the Wheat Powdery Mildew PathogenJournal of fungi (Basel, Switzerland) · 2024Article
- Genetic Diversity Analysis based on the Virulence, Physiology and Regional Variability in Different Isolates of Powdery Mildew in Pea.Journal of fungi (Basel, Switzerland) · 2022Article
- Current Insight into Culture-Dependent and Culture-Independent Methods in Discovering Ascomycetous Taxa.Journal of fungi (Basel, Switzerland) · 2021Review
- Comparative evaluation of different molecular methods for DNA extraction from individual Teladorsagia circumcincta nematodes.BMC biotechnology · 2021Article
- Re-Evaluation of theJournal of fungi (Basel, Switzerland) · 2021Article
- Australia: A Continent Without Native Powdery Mildews? The First Comprehensive Catalog Indicates Recent Introductions and Multiple Host Range Expansion Events, and Leads to the Re-discovery ofFrontiers in microbiology · 2020Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The purpose of this study was to identify a reliable DNA extraction protocol to use on 25-year-old powdery mildew specimens from the reference collection VPRI in order to produce high quality sequences suitable to address taxonomic phylogenetic questions. We tested 13 extraction protocols and two library preparation kits and found the combination of the E.Z.N.A.® Forensic DNA kit for DNA extraction and the NuGen Ovation® Ultralow System library preparation kit was the most suitable for this purpose.
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Registered trials
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