Evidence map›Paper›PMID 32399927›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2020

Purification of Native Fasciola hepatica Fatty Acid-Binding Protein and Induction of Alternative Activation of Human Macrophages.

Olgary Figueroa-Santiago, Ana Espino

Erratum issuedAbstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

2 authors.

Olgary Figueroa-SantiagoDepartment of Biology, University of Puerto Rico at Cayey, Cayey Puerto Rico, USA. olgary.figueroa@upr.edu.
Ana EspinoDepartment of Biology, University of Puerto Rico at Cayey, Cayey Puerto Rico, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This chapter presents the different techniques to purify the native forms of Fasciola hepatica fatty acid-binding protein (Fh12) using size exclusion chromatography and isoelectric focusing (IEF). Also, it presents the procedure to study the immunological effect of the purified protein Fh12 using monocyte-derived macrophages (MDM) obtained from healthy human donors. For this purpose, I present the procedure to isolate and culture peripheral blood mononuclear cells (PBMCs) to generate alternatively activated macrophages (AAMΦ) by in vitro exposure to Fh12.

Indexed as

AnimalsFasciola hepaticaFascioliasisFatty Acid-Binding ProteinsHelminth ProteinsHumansIsoelectric FocusingLeukocytes, MononuclearMacrophagesMonocytesFatty Acid-Binding ProteinsHelminth ProteinsAlternative activated macrophagesCell cultureEndotoxin removalFasciola hepaticaFatty acid-binding proteinIon exchange chromatographyMonocyte-derived macrophagesSize exclusion chromatography

Identifiers

PMID32399927

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.