Evidence map›Paper›PMID 32399807›Full record

ArticleMolecular biology reports2020

Gene expression in human mesenchymal stem cell aging cultures: modulation by short peptides.

Vasily Ashapkin, Vladimir Khavinson, Gregory Shilovsky, Natalia Linkova, Boris Vanuyshin

Abstract read
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In one paragraph

Article in Molecular biology reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed, 1 pooled it
1.3field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 1 synthesis or guideline pooled it, 17 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Peptide Regulation of Chondrogenic Stem Cell Differentiation.International journal of molecular sciences · 2023
    Review
  4. Article
  5. Review
  6. Article
  7. Thymodepressin-Unforeseen Immunosuppressor.Molecules (Basel, Switzerland) · 2021
    Article
  8. Article
  9. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Vasily AshapkinBelozersky Institute of Physical and Chemical Biology, Lomonosov Moscow State University, Moscow, Russia.
Vladimir KhavinsonDepartment of Biogerontology, Saint Petersburg Institute of Bioregulation and Gerontology, St. Petersburg, Russia.
Gregory ShilovskyBelozersky Institute of Physical and Chemical Biology, Lomonosov Moscow State University, Moscow, Russia.
Natalia LinkovaDepartment of Biogerontology, Saint Petersburg Institute of Bioregulation and Gerontology, St. Petersburg, Russia. miayy@yandex.ru.ORCID http://orcid.org/0000-0001-5156-5421
Boris VanuyshinBelozersky Institute of Physical and Chemical Biology, Lomonosov Moscow State University, Moscow, Russia.
Lomonosov Moscow State University · RUSt. Petersburg Institute of Bioregulation and Gerontology · RU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Effects of the short peptides Ala-Glu-Asp (AED), Lys-Glu-Asp (KED) and Lys-Glu (KE) on the expression of IGF1, FOXO1, TERT, TNKS2, and NFκB genes were studied in human embryo bone marrow mesenchymal stem cells (line FetMSCs) variously aged in "passages" or "stationary" cultures. Both cell aging models were similar in gene expression. The main difference was in the TERT gene expression level, which showed an eightfold increase at the "stationary" aging. IGF1 gene expression levels were very similar in both cell culture aging models, being enhanced by 3.5-5.6 fold upon the addition of the peptides. The FOXO1 gene was expressed twice more actively in the "stationary" than in the "passages" aging model. KED peptide inhibited FOXO1 gene expression by 1.6-2.3 fold. KE peptide increased FOXO1 gene expression by about two-fold in the "stationary" aging model but did not affect it in the "passage" aging model. The most striking difference in the peptide effect on cell aging between "passages" and "stationary" aging models was in the KED effects on TNKS2 gene expression; this expression was inhibited by KED in the "passages" model, while stimulation was observed in the "stationary" model. AED, KED, and KE stimulated expression of the NFκB gene in both models. Thus, the peptides studied at nanomolar concentrations modulate the expression of some genes known to be involved in cell aging.

Indexed as

AgingCell DifferentiationCells, CulturedCellular SenescenceDipeptidesForkhead Box Protein O1Gene ExpressionGene Expression Regulation, DevelopmentalHumansInsulin-Like Growth Factor IMesenchymal Stem CellsNF-kappa BOligopeptidesPeptidesTankyrasesTelomerasealanyl-glutamyl-aspartic acidDipeptidesForkhead Box Protein O1FOXO1 protein, humanIGF1 protein, humanInsulin-Like Growth Factor Ilysylglutamic acidlysyl-glutamyl-aspartic acidNF-kappa BOligopeptidesPeptidesTankyrasesTelomeraseTERT protein, humanTNKS2 protein, humanCell agingGenesHuman mesenchymal stem cellsShort peptides

Identifiers

PMID32399807
OpenAlexW3024196022

What OpenQuestion holds

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Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.