Evidence map›Paper›PMID 32399746›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

A Co-culture Model to Study the Effect of Kidney Fibroblast-p90RSK on Epithelial Cell Survival.

Ling Lin, Samantha White, Kebin Hu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Ling LinDepartment of Medicine, The Pennsylvania State University College of Medicine, Hershey, PA, USA. llin1@pennstatehealth.psu.edu.
Samantha WhiteDepartment of Medicine, The Pennsylvania State University College of Medicine, Hershey, PA, USA.
Kebin HuDepartment of Medicine, The Pennsylvania State University College of Medicine, Hershey, PA, USA. kebinhu@pennstatehealth.psu.edu.

Funding

p90 Ribosomal S6 Kinase and Chronic Kidney DiseaseR01DK102624 · NIDDK · PENNSYLVANIA STATE UNIV HERSHEY MED CTR · PI HU, KEBIN · 2014 to 2018
$1.5M
NIDDK NIH HHS R01 DK102624
6 · The paper itself

Abstract

Methods for the mechanistic investigations on renal fibrosis have long been concentrated on individual type of cells, such as fibroblasts and epithelial cells. However, in recent years, growing numbers of studies have been shifting toward the role of the intercellular interactions, such as communication between tubular epithelial cells and fibroblasts. Various co-culture models have been utilized in the studies of cell-cell communication and interaction. In this chapter, we describe an innovative co-culture model employing the porous membranes for spatially partitioning the cells while allowing direct crosstalk between fibroblasts and epithelial cells in an effort of mimicking in vivo environment.

Indexed as

Coculture TechniquesAnimalsCells, CulturedCell SurvivalEpithelial CellsFibroblastsHumansKidneyMiceMice, TransgenicModels, BiologicalRibosomal Protein S6 Kinases, 90-kDaRibosomal Protein S6 Kinases, 90-kDaApoptosisCo-cultureEpithelial cellIn vitrop90RSKPrimary kidney fibroblast

Identifiers

PMID32399746
PMCPMC8429804

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.