ArticleAnimal reproduction2020
Effects of the cryopreservation process on dog sperm integrity.
Article in Animal reproduction, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
8 citing papers in PubMed, 28 citations in OpenAlex.
- Red LED Treatment Confers Superior Early Post-Thaw Sperm Kinetic Resilience and Identifies Progressive Motility as the Most Informative Functional Marker in Dogs.Reproduction in domestic animals = Zuchthygiene · 2026Article
- Post-Ejaculatory Blood Plasma Canine Prostate-Specific Esterase Concentrations May Predict Total Motility Decline After Sperm Freezing in Dogs.Animals : an open access journal from MDPI · 2026Article
- Enhancement of pooled chilled semen quality in four Kintamani dogs through extenders enriched with different concentrations ofJournal of advanced veterinary and animal research · 2026Article
- Addition of low concentration of cholesterol-loaded cyclodextrin (CLC) has a positive effect on cryopreserved canine spermatozoa evaluated by andrological and biophysical methods.BMC veterinary research · 2024Article
- Supplementation of melatonin to cooling and freezing extenders improves canine spermatozoa quality measures.BMC veterinary research · 2022Article
- Photoperiod and Melatonin Supplementation: Variable Effects on the Quality of Chilled Dog Semen.Frontiers in veterinary science · 2022Article
- Overview on the Antioxidants, Egg Yolk Alternatives, and Mesenchymal Stem Cells and Derivatives Used in Canine Sperm Cryopreservation.Animals : an open access journal from MDPI · 2021Review
- Conditioned Medium from Canine Amniotic Membrane-Derived Mesenchymal Stem Cells Improved Dog Sperm Post-Thaw Quality-Related Parameters.Animals : an open access journal from MDPI · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sperm cryopreservation has become an indispensable tool in reproductive biology. However, frozen/thawed semen has a short lifespan due to loss of sperm cell integrity. To better understand which sperm cell structures are compromised by the cryopreservation process and apoptosis markers, the sperm of five healthy mature dogs was analyzed in this study. Analysis was performed after collection, cooling, and thawing via computer assisted sperm analyzer (CASA) and evaluation of membrane fluidity and permeability, phosphatidylserine translocation (Annexin V), membrane integrity, mitochondrial membrane potential, membrane lipid peroxidation (LPO) and activity of the apoptotic markers caspases 3 and 7 by flow cytometry. Cryopreservation decreased total and progressive motility and the percentage of rapid sperm (
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.