Observational studyNature medicine2020
A serological assay to detect SARS-CoV-2 seroconversion in humans.
Registry-linked trialAbstract readObservational Study
In one paragraph
Observational study in Nature medicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT04502472 (Treatment of Severe Coronavirus Disease 2019), which is not on this map. Cited by 1,195 papers, 1 of them a synthesis that pooled it.
0numbers the graph read from it
0cells of the map it votes in
1,195citing papers in PubMed, 1 pooled it
–field-weighted citation impact
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Treatment of Severe Coronavirus Disease 2019 (COVID-19) With Convalescent Plasma Collected From Individuals With Documented Infection and Recovery From COVID-19 (SARS-CoV-2)
Who cites it
1,195 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Immunogenicity and Protective Efficacy of Nucleic Acid-Based Vaccines Against COVID-19: A Systematic Review.Molecular biotechnology · 2024Pooled it
- Systemic and Mucosal Antibody Responses Against Neisseria gonorrhoeae (Ng) Outer Membrane Vesicle Antigens following Four-Component Meningococcal Serogroup B (4CMenB) Immunization in Healthy Adults: A Randomized Phase II Trial.Nature communications · 2026Trial
- Outpatient COVID-19 convalescent plasma recipient antibody thresholds correlated to reduced hospitalizations within a randomized trial.JCI insight · 2024Trial
- The immunogenicity of an extended dosing interval of BNT162b2 against SARS-CoV-2 Omicron variant among healthy school-aged children, a randomized controlled trial.International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases · 2023Trial
- Application of glutamine metabolism-related gene signatures in the non-invasive diagnosis of coronary artery disease: integrating bulk and single-cell transcriptomics.Journal of bioenergetics and biomembranes · 2026Article
- Beyond the blood: Tissue-resident immunity shapes SARS-CoV-2 vaccine antibody responses.bioRxiv : the preprint server for biology · 2026Article
- Human primary antibody response to vaccination follows a partially sequential class-switching program with a checkpoint at IGHG2.Cell reports. Medicine · 2026Article
- Evaluating Memory B Cell Cross-Reactivity Between Ancestral and Future SARS-CoV-2 Variants-Evidence for Original Antigenic Sin.Vaccines · 2026Article
- Yeast Display Technology Enables Rapid Discovery of Low-Nanomolar Macrocyclic Peptide Inhibitors of Human Angiotensin-Converting Enzyme 2.Journal of medicinal chemistry · 2026Article
- Exploring novel kinetics of automated HMicrobiology spectrum · 2026Article
- SARS-CoV-2 Infection and COVID-19 Vaccine Antibody Responses in Two Canadian Cohorts of Persons Living with HIV.Antibodies (Basel, Switzerland) · 2026Article
- Conformational dynamics of SARS-CoV-2 spike on a membrane reveals the allosteric effects of furin cleavage and the D614G mutation.Science advances · 2026Article
- Infection-acquired protection against SARS-CoV-2 infection and clinical severity by number of prior infections.Nature communications · 2026Article
- Integrated Laboratory Evaluation of Rift Valley Fever Virus Antibodies Using the Competitive ELISA and Virus Neutralization Test.Pathogens (Basel, Switzerland) · 2026Article
- Harmonization of IgG antibody values against the SARS-CoV-2 Spike protein.Scientific reports · 2026Article
- Do-it-yourself protein arrays as high-throughput immunoassays enabled by confined droplets on patterned plasmonic chips.Nature communications · 2026Article
- Article
- Adapting antibody-invertase fusion protein immunoassays to multiwell plates for infectious disease antibody quantification.Sensors & diagnostics · 2026Article
- Machine learning models predict long COVID outcomes based on baseline clinical and immunologic factors.Communications medicine · 2026Article
- Article
1,135 more citing papers are in PubMed but not listed here.
Corrections and comments
- Commented on by
- Update of
Authors and funding
30 authors.
Fatima AmanatDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.ORCID http://orcid.org/0000-0002-8029-8227
Daniel StadlbauerDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.ORCID http://orcid.org/0000-0001-6792-7650
Shirin StrohmeierDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Thi H O NguyenDepartment of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, Victoria, Australia.ORCID http://orcid.org/0000-0002-9294-7693
Veronika ChromikovaDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Meagan McMahonDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Kaijun JiangDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Guha Asthagiri ArunkumarDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.ORCID http://orcid.org/0000-0003-3665-2708
Denise JurczyszakDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Jose PolancoDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Maria Bermudez-GonzalezDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Giulio KleinerDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.ORCID http://orcid.org/0000-0002-1830-3961
Teresa AydilloDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Lisa MiorinDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Daniel S FiererDivision of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA.ORCID http://orcid.org/0000-0002-3427-8022
Luz Amarilis LugoDivision of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Erna Milunka KojicDivision of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Jonathan StoeverDivision of Pulmonary, Critical Care, and Sleep Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA.ORCID http://orcid.org/0000-0003-1995-101X
Sean T H LiuDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Charlotte Cunningham-RundlesDivision of Clinical Immunology, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Philip L FelgnerDepartment of Physiology and Biophysics, University of California, Irvine, Irvine, CA, USA.
Thomas MoranDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Adolfo García-SastreDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.ORCID http://orcid.org/0000-0002-6551-1827
Daniel CaplivskiTravel Medicine Program, Division of Infectious Diseases, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Allen C ChengSchool of Public Health and Preventive Medicine, Monash University and Infection Prevention and Healthcare Epidemiology Unit Alfred Health, Melbourne, Victoria, Australia.
Katherine KedzierskaDepartment of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, Victoria, Australia.ORCID http://orcid.org/0000-0001-6141-335X
Olli VapalahtiDepartment of Virology, Medicum, University of Helsinki, Helsinki, Finland.
Jussi M HepojokiDepartment of Virology, Medicum, University of Helsinki, Helsinki, Finland.ORCID http://orcid.org/0000-0001-5699-214X
Viviana SimonDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Florian KrammerDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA. florian.krammer@mssm.edu.ORCID http://orcid.org/0000-0003-4121-776X
Funding
COLLABORATIVE INFLUENZA VACCINE INNOVATION CENTER: UNIVERSAL INFLUENZA VACCINE RESEARCH75N93019C00051 · NIAID · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI KRAMMER, FLORIAN · 2019 to 2025
$105.4MRegulation of IgA class switching in the intestinal mucosaP01AI061093 · NIAID · MOUNT SINAI SCHOOL OF MEDICINE OF NYU · PI CUNNINGHAM-RUNDLES, CHARLOTTE · 2004 to 2021
$29.8MMSHS Translational Science HubUL1TR001433 · NCATS · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI WRIGHT, ROSALIND J · 2015 to 2019
$26.6MResources to Assist Investigations in Primary Immunodeficiency Diseases (U24)U24AI086037 · NIAID · IMMUNE DEFICIENCY FOUNDATION · PI CUNNINGHAM-RUNDLES, CHARLOTTE · 2010 to 2019
$6.5MAcademy of Finland (Suomen Akatemia) TYH2018322Department of Health | National Health and Medical Research Council (NHMRC) 1071916Department of Health | National Health and Medical Research Council (NHMRC) 1102792NCATS NIH HHS UL1 TR001433NIAID NIH HHS 75N93019C00051NIAID NIH HHS HHSN272201400008CNIAID NIH HHS P01 AI061093NIAID NIH HHS U24 AI086037
Abstract
Here, we describe a serological enzyme-linked immunosorbent assay for the screening and identification of human SARS-CoV-2 seroconverters. This assay does not require the handling of infectious virus, can be adjusted to detect different antibody types in serum and plasma and is amenable to scaling. Serological assays are of critical importance to help define previous exposure to SARS-CoV-2 in populations, identify highly reactive human donors for convalescent plasma therapy and investigate correlates of protection.
Indexed as
SeroconversionAdultBetacoronavirusCase-Control StudiesClinical Laboratory TechniquesCoronavirus InfectionsCOVID-19COVID-19 SerotherapyCOVID-19 TestingElectrophoresis, Polyacrylamide GelEnzyme-Linked Immunosorbent AssayHumansImmunization, PassiveLongitudinal StudiesMiddle AgedNeutralization Tests
Identifiers
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Read underepoch 390
Registered trials
NCT04502472ClinicalTrials.gov
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.