Evidence map›Paper›PMID 32398689›Full record

ArticleScientific reports2020

Fourier-transform infrared spectroscopy for monitoring proteolytic reactions using dry-films treated with trifluoroacetic acid.

Kenneth Aase Kristoffersen, Aart van Amerongen, Ulrike Böcker, Diana Lindberg, Sileshi Gizachew Wubshet, Heleen de Vogel-van den Bosch, Svein Jarle Horn, Nils Kristian Afseth

Abstract read
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In one paragraph

Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Article
  2. A Solution-Based Deposition Method Enabling Pigment Blue Edible Electrochemical Transistors.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025
    Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Plants (Basel, Switzerland) · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Kenneth Aase KristoffersenNofima - Norwegian Institute of Food, Fisheries and Aquaculture Research, P.O. Box 210 N-1431, Ås, Norway. kenneth.kristoffersen@nofima.no.
Aart van AmerongenBioSensing & Diagnostics, Wageningen Food & Biobased Research, Wageningen University & Research, Bornse Weilanden 9, 6708 WG, Wageningen, the Netherlands.
Ulrike BöckerNofima - Norwegian Institute of Food, Fisheries and Aquaculture Research, P.O. Box 210 N-1431, Ås, Norway.
Diana LindbergNofima - Norwegian Institute of Food, Fisheries and Aquaculture Research, P.O. Box 210 N-1431, Ås, Norway.
Sileshi Gizachew WubshetNofima - Norwegian Institute of Food, Fisheries and Aquaculture Research, P.O. Box 210 N-1431, Ås, Norway.
Heleen de Vogel-van den BoschBioSensing & Diagnostics, Wageningen Food & Biobased Research, Wageningen University & Research, Bornse Weilanden 9, 6708 WG, Wageningen, the Netherlands.
Svein Jarle HornFaculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (NMBU), P.O. Box 5003, N-1432, Ås, Norway.
Nils Kristian AfsethNofima - Norwegian Institute of Food, Fisheries and Aquaculture Research, P.O. Box 210 N-1431, Ås, Norway.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In this study we explore the potential of using Fourier-transform infrared (FTIR) spectra of trifluoroacetate-protein and peptide complexes for monitoring proteolytic reactions. The idea of treating dry-films of protein hydrolysates with trifluoroacetic acid (TFA) prior to FTIR analysis is based on the unique properties of TFA. By adding a large excess of TFA to protein hydrolysate samples, the possible protonation sites of the proteins and peptides will be saturated. In addition, TFA has a low boiling point when protonated as well as complex-forming abilities. When forming TFA-treated dry-films of protein hydrolysates, the excess TFA will evaporate and the deprotonated acid (CF

Identifiers

PMID32398689
PMCPMC7217958

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.