Evidence map›Paper›PMID 32398422›Full record

ArticleCornea2020

Metagenomic Deep Sequencing to Investigate for an Infectious Etiology of Iridocorneal Endothelial Syndrome.

Plern Sutra, Jennifer Rose-Nussbaumer, John A Gonzales, Kaidi Wang, Armin Hinterwirth, Gerami Seitzman, Michele Bloomer, Nisha Acharya, Thuy Doan

Open access · greenAbstract readCase Reports
PubMed Full text at PMC Publisher
In one paragraph

Article in Cornea, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 53% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 2 countries.

Plern SutraFrancis I. Proctor Foundation, University of California, San Francisco, San Francisco, CA.
Jennifer Rose-NussbaumerFrancis I. Proctor Foundation, University of California, San Francisco, San Francisco, CA.
John A GonzalesFrancis I. Proctor Foundation, University of California, San Francisco, San Francisco, CA.
Kaidi WangFrancis I. Proctor Foundation, University of California, San Francisco, San Francisco, CA.
Armin HinterwirthFrancis I. Proctor Foundation, University of California, San Francisco, San Francisco, CA.
Gerami SeitzmanFrancis I. Proctor Foundation, University of California, San Francisco, San Francisco, CA.
Michele BloomerDepartment of Ophthalmology, University of California, San Francisco, San Francisco, CA; and.
Nisha AcharyaFrancis I. Proctor Foundation, University of California, San Francisco, San Francisco, CA.
Thuy DoanFrancis I. Proctor Foundation, University of California, San Francisco, San Francisco, CA.
San Francisco Foundation · USUniversity of California, San Francisco · US

Funding

Ocular Surface Microbiome, Virome, and Pathogens in Ophthalmic Disease and HealthK08EY026986 · NEI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI DOAN, THUY A · 2016 to 2020
$1.2M
NEI NIH HHS K08 EY026986
6 · The paper itself

Abstract

purposeIridocorneal endothelial (ICE) syndrome is a group of rare ocular conditions that result from abnormal corneal endothelial cells, leading to secondary glaucoma, iris distortions, and corneal edema. The etiology of ICE is unknown, although it has been associated with viral infections, such as herpes simplex virus. In this study, we sought to identify an infectious etiology for ICE using advanced molecular techniques.

methodsMetagenomic RNA sequencing (MDS) is a high-throughput sequencing approach that can identify all pathogens in any clinical sample, including RNA viruses. Descemet membrane and aqueous fluid from patients with ICE syndrome were subjected to MDS testing.

resultsSamples from 3 patients with ICE were analyzed. MDS was performed on the aqueous fluid of 3 patients and Descemet membrane and endothelial cell tissue from 1 patient. Viral pathogens were not identified in any of the samples.

conclusionsWe were unable to identify a viral etiology in the tissues of patients with the Chandler variant of ICE syndrome, although this study was limited by sample size.

Indexed as

MetagenomicsAqueous HumorCytomegalovirusDescemet MembraneEndothelium, CornealEye Infections, ViralFemaleHerpesvirus 3, HumanHigh-Throughput Nucleotide SequencingHumansIridocorneal Endothelial SyndromeMaleMiddle AgedRetrospective StudiesRNA, ViralSimplexvirusRNA, Viral

Identifiers

PMID32398422
PMCPMC7483698
OpenAlexW3025121312

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.