ArticleBMC plant biology2020
One-step generation of composite soybean plants with transgenic roots by Agrobacterium rhizogenes-mediated transformation.
Article in BMC plant biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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59 citing papers in PubMed.
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- The Genetic Transformation Mechanism and Application of Plant Hairy Roots.Plants (Basel, Switzerland) · 2026Review
- Reducing recalcitrance of black pepper toHorticulture research · 2026Article
- A RUBY-based in vivo hairy root induction system for rapid assessment of transformant and genome editing efficiency in potato.Journal, genetic engineering & biotechnology · 2026Article
- Efficient prime editors for heritable multiplex precision genome editing in soybean.Nature plants · 2026Article
- GmSNAP14: a key contributor to soybean cyst nematode resistance in soybean.The New phytologist · 2026Article
- A Rapid and Visual Soybean Hairy Root Transformation Protocol Using the RUBY Reporter.Bio-protocol · 2026Article
- Scalable production of soybean hairy roots: a reliable method for field trials.Plant cell reports · 2026Article
- Transposon-derived genome editors in plants: from compact nucleases to large-fragment integration and regeneration strategies.Engineering in life sciences · 2026Review
- Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Functional divergence of two soybean cytosolic serine hydroxymethyltransferases in development and defense against soybean cyst nematode.Frontiers in plant science · 2026Article
- A flanking-nicks prime editor (FLICK-PE) system to boost prime editing in dicots.Nature communications · 2025Article
- Characterization of the Soybean (Plants (Basel, Switzerland) · 2025Article
- Improved rapid and efficient hairy root transformation usingPlant biotechnology (Tokyo, Japan) · 2025Article
- High-Frequency Generation of Homozygous/Biallelic Mutants via CRISPR/Cas9 Driven byInternational journal of molecular sciences · 2025Article
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- pMAGs: A Versatile and Efficient Vector System for Multi-Gene Studies in Plants.Plants (Basel, Switzerland) · 2025Article
- Soybean Cyst Nematode-Resistant Protein AATPlant direct · 2025Article
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6 authors.
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Abstract
backgroundAgrobacterium rhizogenes-mediated (ARM) transformation is a highly efficient technique for generating composite plants composed of transgenic roots and wild-type shoot, providing a powerful tool for studying root biology. The ARM transformation has been established in many plant species, including soybean. However, traditional transformation of soybean, transformation efficiency is low. Additionally, the hairy roots were induced in a medium, and then the generated composite plants were transplanted into another medium for growth. This two-step operation is not only time-consuming, but aggravates contamination risk in the study of plant-microbe interactions.
resultsHere, we report a one-step ARM transformation method with higher transformation efficiency for generating composite soybean plants. Both the induction of hairy roots and continuous growth of the composite plants were conducted in a single growth medium. The primary root of a 7-day-old seedling was decapitated with a slanted cut, the residual hypocotyl (maintained 0.7-1 cm apical portion) was inoculated with A. rhizogenes harboring the gene construct of interest. Subsequently, the infected seedling was planted into a pot with wet sterile vermiculite. Almost 100% of the infected seedlings could produce transgenic positive roots 16 days post-inoculation in 7 tested genotypes. Importantly, the transgenic hairy roots in each composite plant are about three times more than those of the traditional ARM transformation, indicating that the one-step method is simpler in operation and higher efficiency in transformation. The reliability of the one-step method was verified by CRISPR/Cas9 system to knockout the soybean Rfg1, which restricts nodulation in Williams 82 (Nod-) by Sinorhizobium fredii USDA193. Furthermore, we applied this method to analyze the function of Arabidopsis YAO promoter in soybean. The activity of YAO promoter was detected in whole roots and stronger in the root tips. We also extended the protocol to tomato.
conclusionsWe established a one-step ARM transformation method, which is more convenient in operation and higher efficiency (almost 100%) in transformation for generating composite soybean plants. This method has been validated in promoter functional analysis and rhizobia-legume interactions. We anticipate a broad application of this method to analyze root-related events in tomato and other plant species besides soybean.
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