Evidence map›Paper›PMID 32397678›Full record

ArticleBiomolecules2020

Blueberry-Derived Exosome-Like Nanoparticles Counter the Response to TNF-α-Induced Change on Gene Expression in EA.hy926 Cells.

Mariangela De Robertis, Angelo Sarra, Valentina D'Oria, Francesco Mura, Federico Bordi, Paolo Postorino, Deborah Fratantonio

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomolecules, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 103 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
103citing papers in PubMed, 1 pooled it
6.1field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

103 citing papers in PubMed, 1 synthesis or guideline pooled it, 166 citations in OpenAlex.

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43 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 1 country.

Mariangela De RobertisInstitute of Biomembranes, Bioenergetics and Molecular Biotechnologies, Consiglio Nazionale delle Ricerche (CNR), 70126 Bari, Italy.ORCID 0000-0002-5409-639X
Angelo SarraDepartment of Science, University of Roma Tre, 00145 Rome, Italy.
Valentina D'OriaBambino Gesù Children's Hospital IRCCS, Research Laboratories, 00146 Rome, Italy.
Francesco MuraCenter for Nanotechnology for Engineering (CNIS), Sapienza University of Rome, 00185 Rome, Italy.
Federico BordiCNR-ISC UOS Sapienza, Sapienza University of Rome, 00185 Rome, Italy.
Paolo PostorinoDepartment of Physics, Sapienza University of Rome, 00185 Rome, Italy.ORCID 0000-0002-3809-0676
Deborah FratantonioDepartment of Biosciences, Biotechnology and Biopharmaceutics, University of Bari Aldo Moro, 70125 Bari, Italy.ORCID 0000-0003-4835-0571
Sapienza University of Rome · ITBambino Gesù Children's Hospital · ITInstitute of Biomembranes, Bioenergetics and Molecular Biotechnologies · ITRoma Tre University · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Exosome-like nanoparticles (ELNs) are attracting interest as important vehicles of intercellular communication, both in prokaryotes and eukaryotes. Recently, dietary nanoparticles similar to mammalian exosomes have attracted attention for these features. In particular they appear to be relevant in the modulation of several cellular processes as well as candidate carriers of bioactive molecules (proteins, lipids, and nucleic acids, including miRNAs) with therapeutic value. Herein, we investigated the cellular uptake of blueberry-derived ELNs (B-ELNs) by a human stabilized endothelial cell line (EA.hy926) and the ability of B-ELNs to modulate the expression of inflammatory genes as the response of tumor necrosis factor-α (TNF-α). Our results indicate that 1) EA.hy926 cells internalize B-ELNs in a dose-dependent manner; 2) pretreatment with B-ELNs counters TNF-α-induced reactive oxygen species (ROS) generation and loss of cell viability and modulates the differential expression of 29 genes (fold change > 1.5) induced by TNF-α compared to control; 3) pathway analysis reveals their involvement in a total of 340 canonical pathways, 121 KEGG pathways, and 121 GO Biological processes; and 4) the intersection between differentially expressed (DE) genes and miRNAs contained in B-ELNs unveils a set of candidate target genes, such as prostaglandin I2 synthase (PTGIS), mitogen-activated protein kinase 14 (MAPK14), and phosphodiesterase 7A (PDE7A), for ELNs-contained cargo. In conclusion, our study indicates that B-ELNs can be considered candidate therapeutic carriers of bioactive compounds potentially able to protect vascular system against various stressors.

Indexed as

Base SequenceBlueberry PlantsCell DeathCell LineCell SurvivalCytoprotectionEndocytosisExosomesGene Expression RegulationGene OntologyHumansInflammationMicroRNAsNanoparticlesOxidative StressReactive Oxygen SpeciesMicroRNAsReactive Oxygen SpeciesRNA, MessengerTumor Necrosis Factor-alphablueberrycross-kingdomexosome-like nanoparticlesgene expressioninflammationoxidative stresspathway analysisuptake

Identifiers

PMID32397678
PMCPMC7277966
OpenAlexW3021615011

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.