ArticleMicromachines2020
Single Cell Hydrodynamic Stretching and Microsieve Filtration Reveal Genetic, Phenotypic and Treatment-Related Links to Cellular Deformability.
Article in Micromachines, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
6 citing papers in PubMed.
- A Microfluidic Approach for Assessing the Rheological Properties of Healthy Versus Thalassemic Red Blood Cells.Micromachines · 2025Article
- The drug resistance feature of acute myeloid leukemia is related to the cell stiffness.Biophysics reviews · 2025Article
- Review
- Correlating mechanical and gene expression data on the single cell level to investigate metastatic phenotypes.iScience · 2023Article
- Cancer-on-a-Chip: Models for Studying Metastasis.Cancers · 2022Review
- Editorial for the Special Issue on Micro/Nanofluidic Devices for Single Cell Analysis, Volume II.Micromachines · 2021Article
Corrections and comments
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Authors and funding
5 authors.
Funding
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Abstract
Deformability is shown to correlate with the invasiveness and metastasis of cancer cells. Recent studies suggest epithelial-to-mesenchymal transition (EMT) might enable cancer metastasis. However, the correlation of EMT with cancer cell deformability has not been well elucidated. Cellular deformability could also help evaluate the drug response of cancer cells. Here, we combine hydrodynamic stretching and microsieve filtration to study cellular deformability in several cellular models. Hydrodynamic stretching uses extensional flow to rapidly quantify cellular deformability and size with high throughput at the single cell level. Microsieve filtration can rapidly estimate relative deformability in cellular populations. We show that colorectal cancer cell line RKO with the mesenchymal-like feature is more flexible than the epithelial-like HCT116. In another model, the breast epithelial cells MCF10A with deletion of the TP53 gene are also significantly more deformable compared to their isogenic wildtype counterpart, indicating a potential genetic link to cellular deformability. We also find that the drug docetaxel leads to an increase in the size of A549 lung cancer cells. The ability to associate mechanical properties of cancer cells with their phenotypes and genetics using single cell hydrodynamic stretching or the microsieve may help to deepen our understanding of the basic properties of cancer progression.
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Registered trials
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