ArticleAnimals : an open access journal from MDPI2020
Isolation and Identification of Bovine Preadipocytes and Screening of MicroRNAs Associated with Adipogenesis.
Article in Animals : an open access journal from MDPI, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 46 citations in OpenAlex.
- Effects of dietary glutamine supplementation on lipid metabolism, fatty acid composition, and taste properties in the longissimus thoracis muscle of feed-restricted yaks.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2026Article
- Multi-omics dissection of the genetic regulation underlying fatty acid composition in cattle.Genome biology · 2025Article
- Exploring the regulatory mechanisms of differentially expressed circRNAs during bovine intramuscular adipogenic differentiation.BMC genomics · 2025Article
- Unravelling bovine preadipocyte differentiation and their three-dimensional cultivation for cellular agriculture.NPJ science of food · 2025Article
- Expression of miRNA in the Semitendinosus Muscle of Cattle Breeds with Varying Intramuscular Fat Deposition.Genes · 2025Article
- Mechanisms of Adipose Tissue Metabolism in Naturally Grazing Sheep at Different Growth Stages: Insights from mRNA and miRNA Profiles.International journal of molecular sciences · 2025Article
- Multiple strategies association revealed functional candidate FASN gene for fatty acid composition in cattle.Communications biology · 2025Article
- Neuron-derived neurotrophic factor promotes the differentiation of intramuscular and subcutaneous adipocytes in goat.Animal biotechnology · 2024Article
- Oleanolic acid attenuates obesity through modulating the lipid metabolism in high-fat diet-fed mice.Food science & nutrition · 2024Article
- Effect of breed and dietary composition on the miRNA profile of beef steers divergent for feed efficiency.Scientific reports · 2024Article
- Mining key circRNA-associated-ceRNA networks for milk fat metabolism in cows with varying milk fat percentages.BMC genomics · 2024Article
- CircRNA-5335 Regulates the Differentiation and Proliferation of Sheep Preadipocyte via the miR-125a-3p/STAT3 Pathway.Veterinary sciences · 2024Article
- Long non-coding RNA (LncRNA) and epigenetic factors: their role in regulating the adipocytes in bovine.Frontiers in genetics · 2024Review
- Effect of miR-149-5p on intramuscular fat deposition in pigs based on metabolomics and transcriptomics.BMC genomics · 2023Article
- Excavation and characterization of key circRNAs for milk fat percentage in Holstein cattle.Journal of animal science · 2023Article
- Unlocking the potential of stem cells: Their crucial role in the production of cultivated meat.Current research in food science · 2023Review
- CRISPR/Cas9-Induced Knockout of miR-24 Reduces Cholesterol and Monounsaturated Fatty Acid Content in Primary Goat Mammary Epithelial Cells.Foods (Basel, Switzerland) · 2022Article
- miR-24-3p Dominates the Proliferation and Differentiation of Chicken Intramuscular Preadipocytes by BlockingGenes · 2022Article
- Review
- MicroRNA and circular RNA profiling in the deposited fat tissue of Sunite sheep.Frontiers in veterinary science · 2022Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
The elucidation of the mechanisms of preadipocyte differentiation and fat accumulation in adipocytes is a major work in beef cattle breeding. As important post-transcriptional regulators, microRNAs (miRNAs) take part in cell proliferation, differentiation, apoptosis, and fat metabolism through binding seed sites of targeting mRNAs. The aim of this study was to isolate and identify bovine preadipocytes and screen miRNAs associated with adipogenesis. Bovine preadipocytes were isolated from subcutaneous fatty tissue and induced to differentiate into adipocytes. Verification of preadipocytes and adipocytes was performed by qRT-PCR (real-time quantitative reverse transcription PCR), Oil Red O staining, and immunofluorescence staining. Total RNA was extracted for small RNA sequencing. The sequencing data showed that 131 miRNAs were highly expressed in adipocytes, and 119 miRNAs were highly expressed in preadipocytes. Stem-loop qPCR (stem-loop quantitative real-time PCR) results showed that the expression patterns of 11 miRNAs were consistent with the sequencing results (miR-149-5p, miR-24-3p, miR-199a-5p, miR-33a, etc.). According to KEGG pathway and Gene Ontology (GO) analyses, multiple predicted target genes were associated with lipid metabolism. In summary, this study provides a protocol of isolating bovine preadipocytes and screening various differently expressed miRNAs during preadipocyte differentiation.
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