ArticleBMC veterinary research2020
Serological and molecular evidence of Brucella species in the rapidly growing pig sector in Kenya.
Article in BMC veterinary research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it, 23 citations in OpenAlex.
- Beyond Serology: A Meta-Analysis of Advancements in Molecular Detection of Brucella spp. in Seronegative Animals and Biological Samples.Veterinary medicine and science · 2025Pooled it
- Epidemiology of human and animal brucellosis in Kenya: A One Health meta-regression and network analysis.One health (Amsterdam, Netherlands) · 2026Article
- Integrated Surveillance for Human and Animal Brucellosis in Kenya: A Predictive Analysis.Tropical medicine and infectious disease · 2025Article
- Molecular detection, seroprevalence, and phylogenetic analysis ofVeterinary world · 2025Article
- Epidemiology of Swine Brucellosis in Selected Districts of East Shewa Zone, Ethiopia.Veterinary medicine international · 2025Article
- Seroprevalence and risk factors of brucellosis in pastoralists and their livestock in Central Equatoria State, South Sudan.PLoS neglected tropical diseases · 2024Article
- Mapping brucellosis risk in Kenya and its implications for control strategies in sub-Saharan Africa.Scientific reports · 2023Article
- Sero-prevalence and factors associated with anti-Frontiers in epidemiology · 2023Article
- Brucellae as resilient intracellular pathogens: epidemiology, host-pathogen interaction, recent genomics and proteomics approaches, and future perspectives.Frontiers in veterinary science · 2023Review
- Accuracy of molecular diagnostic methods for the detection of bovine brucellosis: A systematic review and meta-analysis.Veterinary world · 2022Article
- Prevalence of brucellosis in livestock keepers and domestic ruminants in Baringo County, Kenya.PLOS global public health · 2022Article
- Molecular epidemiology of Brucella species in mixed livestock-human ecosystems in Kenya.Scientific reports · 2021Article
- Validation of real-time polymerase chain reaction versus conventional polymerase chain reaction for diagnosis of brucellosis in cattle sera.Veterinary world · 2021Article
Corrections and comments
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Authors and funding
15 authors at 8 institutions in 5 countries.
Funding
Abstract
backgroundBrucellosis is an emerging yet neglected zoonosis that has been reported in Kenya. Epidemiological data on brucellosis in ruminants is readily accessible; however, reports on brucellosis in pigs remain limited. This study sought to detect Brucella infection in pig serum by both serological and molecular techniques. Serum from 700 pigs randomly collected at a centralized abattoir in Nairobi region, Kenya were screened in parallel, using both Rose Bengal Test (RBT) and competitive Enzyme-Linked Immuno-sorbent Assay (cELISA) for antibodies against Brucella spp. All sera positive by RBT and 16 randomly selected negative samples were further tested using conventional PCR targeting bcsp31 gene and real-time PCR (RT-PCR) assays targeting IS711 and bcsp31 genes.
resultsA prevalence of 0.57% (n = 4/700) was estimated using RBT; none of these samples was positive on cELISA. All RBT positive sera were also positive by both PCRs, while two sero-negative samples also tested positive on RT-PCR (n = 6/20). Brucella abortus was detected in four out of the six PCR positive samples through a real-time multiplex PCR.
conclusionThe detection of antibodies against Brucella spp. and DNA in serum from slaughterhouse pigs confirm the presence of Brucella in pigs. Therefore, investigation of the epidemiology and role of pigs in the transmission of brucellosis in Kenya is needed. Further targeted studies would be useful to systematically quantify and identify the spp. of Brucella in pigs.
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