ArticleThe Plant journal : for cell and molecular biology2020
Exocyst mutants suppress pollen tube growth and cell wall structural defects of hydroxyproline O-arabinosyltransferase mutants.
Article in The Plant journal : for cell and molecular biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 33 citations in OpenAlex.
- ZmHPATR1 orchestrates phyllosphere organic acid flux to recruit Sphingomonas and enhance resistance of maize to Curvularia leaf spot.The New phytologist · 2026Article
- Unconventional vesicle transport driven by formin-actin-myosin working system during pollen germination.Science advances · 2026Article
- Using Sodium Thiosulfate to Heighten Copper (Cu (II)) Tolerance of the Freshwater MicroalgaBiology · 2026Article
- The RING-Type E3 Ligase BOI Interacts with EXO70E2 and Mediates Its Ubiquitination inLife (Basel, Switzerland) · 2024Article
- The Arabidopsis SNARE complex genes regulate the early stages of pollen-stigma interactions.Plant reproduction · 2024Article
- Investigating a role for PUB17 and PUB16 in the self-incompatibility signaling pathway in transgenicPlant direct · 2024Article
- Phenotypic characterization and candidate gene analysis of a short kernel and brassinosteroid insensitive mutant from hexaploid oat (Frontiers in plant science · 2024Article
- The hydroxyproline O-arabinosyltransferase FIN4 is required for tomato pollen intine development.Plant reproduction · 2023Article
- O-glycosylation of the extracellular domain of pollen class I formins modulates their plasma membrane mobility.Journal of experimental botany · 2022Article
- Let's shape again: the concerted molecular action that builds the pollen tube.Plant reproduction · 2022Review
- SEC1A is a major Arabidopsis Sec1/Munc18 gene in vesicle trafficking during pollen tube tip growth.The Plant journal : for cell and molecular biology · 2022Article
- Autophagy is required for self-incompatible pollen rejection in two transgenic Arabidopsis thaliana accessions.Plant physiology · 2022Article
- Hydroxyproline-Frontiers in plant science · 2022Article
- Functional Specialization within the EXO70 Gene Family in Arabidopsis.International journal of molecular sciences · 2021Article
- A Golgi-localized manganese transporter functions in pollen tube tip growth to control male fertility inPlant communications · 2021Article
- Extensin, an underestimated key component of cell wall defence?Annals of botany · 2021Article
- Extensins: Self-Assembly, Crosslinking, and the Role of Peroxidases.Frontiers in plant science · 2021Review
- Plant ProteinFrontiers in plant science · 2021Review
- EXO70A2 Is Critical for Exocyst Complex Function in Pollen Development.Plant physiology · 2020Article
- Regulation of Exocyst Function in Pollen Tube Growth by Phosphorylation of Exocyst Subunit EXO70C2.Frontiers in plant science · 2020Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
HYDROXYPROLINE O-ARABINOSYLTRANSFERASEs (HPATs) initiate a post-translational protein modification (Hyp-Ara) found abundantly on cell wall structural proteins. In Arabidopsis thaliana, HPAT1 and HPAT3 are redundantly required for full pollen fertility. In addition to the lack of Hyp-Ara in hpat1/3 pollen tubes (PTs), we also found broadly disrupted cell wall polymer distributions, particularly the conversion of the tip cell wall to a more shaft-like state. Mutant PTs were slow growing and prone to rupture and morphological irregularities. In a forward mutagenesis screen for suppressors of the hpat1/3 low seed-set phenotype, we identified a missense mutation in exo70a2, a predicted member of the vesicle-tethering exocyst complex. The suppressed pollen had increased fertility, fewer morphological defects and partially rescued cell wall organization. A transcriptional null allele of exo70a2 also suppressed the hpat1/3 fertility phenotype, as did mutants of core exocyst complex member sec15a, indicating that reduced exocyst function bypassed the PT requirement for Hyp-Ara. In a wild-type background, exo70a2 reduced male transmission efficiency, lowered pollen germination frequency and slowed PT elongation. EXO70A2 also localized to the PT tip plasma membrane, consistent with a role in exocyst-mediated secretion. To monitor the trafficking of Hyp-Ara modified proteins, we generated an HPAT-targeted fluorescent secretion reporter. Reporter secretion was partially dependent on EXO70A2 and was significantly increased in hpat1/3 PTs compared with the wild type, but was reduced in the suppressed exo70a2 hpat1/3 tubes.
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