Evidence map›Paper›PMID 32391350›Full record

ArticleFrontiers in bioengineering and biotechnology2020

Evaluation of Downstream Processing, Extraction, and Quantification Strategies for Single Cell Oil Produced by the Oleaginous Yeasts

Olga Gorte, Rebecca Hollenbach, Ioannis Papachristou, Christian Steinweg, Aude Silve, Wolfgang Frey, Christoph Syldatk, Katrin Ochsenreither

Abstract read
In one paragraph

Article in Frontiers in bioengineering and biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed.

  1. The yeasts associated with the fungus-growing termite, Macrotermes michaelseni, of Botswana: biodiversity and potential functional roles.International microbiology : the official journal of the Spanish Society for Microbiology · 2026
    Article
  2. Article
  3. Review
  4. Article
  5. Review
  6. Review
  7. Article
  8. Article
  9. Global Transcriptome Profile of the Oleaginous YeastJournal of fungi (Basel, Switzerland) · 2021
    Article
  10. Review
  11. Article
  12. Article
  13. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Olga GorteInstitute of Process Engineering in Life Science 2: Technical Biology, Karlsruhe Institute of Technology, Karlsruhe, Germany.
Rebecca HollenbachInstitute of Process Engineering in Life Science 2: Technical Biology, Karlsruhe Institute of Technology, Karlsruhe, Germany.
Ioannis PapachristouInstitute for Pulsed Power and Microwave Technology, Karlsruhe Institute of Technology, Karlsruhe, Germany.
Christian SteinwegInstitute of Process Engineering in Life Science 3: Bioprocess Engineering, Karlsruhe Institute of Technology, Karlsruhe, Germany.
Aude SilveInstitute for Pulsed Power and Microwave Technology, Karlsruhe Institute of Technology, Karlsruhe, Germany.
Wolfgang FreyInstitute for Pulsed Power and Microwave Technology, Karlsruhe Institute of Technology, Karlsruhe, Germany.
Christoph SyldatkInstitute of Process Engineering in Life Science 2: Technical Biology, Karlsruhe Institute of Technology, Karlsruhe, Germany.
Katrin OchsenreitherInstitute of Process Engineering in Life Science 2: Technical Biology, Karlsruhe Institute of Technology, Karlsruhe, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Single cell oil (SCO) produced by oleaginous yeasts is considered as a sustainable source for biodiesel and oleochemicals since its production does not compete with food or feed and high yields can be obtained from a wide variety of carbon sources, e.g., acetate or lignocellulose. Downstream processing is still costly preventing the broader application of SCO. Direct transesterification of freeze-dried biomass is widely used for analytical purposes and for biodiesel production but it is energy intensive and, therefore, expensive. Additionally, only fatty acid esters are produced limiting the subsequent applications. The harsh conditions applied during direct esterification might also damage high-value polyunsaturated fatty acids. Unfortunately, universal downstream strategies effective for all yeast species do not exist and methods have to be developed for each yeast species due to differences in cell wall composition. Therefore, the aim of this study was to evaluate three industrially relevant cell disruption methods combined with three extraction systems for the SCO extraction of two novel, unconventional oleaginous yeasts,

Indexed as

Apiotrichum porosum DSM 27194cell disruptiondownstream processinglipid extractionoleaginous yeastsSaitozyma podzolica DSM 27192single cell oiltransesterification

Identifiers

PMID32391350
PMCPMC7193083

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.