ArticleFrontiers in microbiology2020
Analysis of a Marseillevirus Transcriptome Reveals Temporal Gene Expression Profile and Host Transcriptional Shift.
Article in Frontiers in microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 32 citations in OpenAlex.
- A history of over 40 years of potentially pathogenic free-living amoeba studies in Brazil - a systematic review.Memorias do Instituto Oswaldo Cruz · 2022Pooled it
- Infection cycles of viruses of the phylum Nucleocytoviricota.Nature reviews. Microbiology · 2026Review
- A giant virus forms a specialized subcellular environment within its amoeba host for efficient translation.Nature microbiology · 2026Article
- Evolutionarily conserved grammar rules viral factories of amoeba-infecting members of the hyperdiverseProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Ultrastructural and transcriptional changes during a giant virus infection of a green alga.Npj viruses · 2025Article
- Sputnik virophage disrupts the transcriptional regulation of its host giant virus.Journal of virology · 2025Article
- Proteome-scale structural prediction of the giant Marseillevirus reveals conserved folds and putative homologs of the hypothetical proteins.Archives of virology · 2024Article
- The consequences of viral infection on protists.Communications biology · 2024Review
- Review
- Amoebal Tubulin Cleavage Late during Infection Is a Characteristic Feature ofMicrobiology spectrum · 2022Article
- Giant virus biology and diversity in the era of genome-resolved metagenomics.Nature reviews. Microbiology · 2022Review
- The Astounding World of Glycans from Giant Viruses.Chemical reviews · 2022Review
- Viral histones: pickpocket's prize or primordial progenitor?Epigenetics & chromatin · 2022Review
- Review
- RNA Sequencing of Medusavirus Suggests Remodeling of the Host Nuclear Environment at an Early Infection Stage.Microbiology spectrum · 2021Article
- High Transcriptional Activity and Diverse Functional Repertoires of Hundreds of Giant Viruses in a Coastal Marine System.mSystems · 2021Article
- Article
- Comparative Analysis of Transcriptional Regulation Patterns: Understanding the Gene Expression Profile inPathogens (Basel, Switzerland) · 2021Review
- Metatranscriptome Library Preparation Influences Analyses of Viral Community Activity During a Brown Tide Bloom.Frontiers in microbiology · 2021Article
- Marseilleviruses: An Update in 2021.Frontiers in microbiology · 2021Review
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Marseilleviruses comprise a family of large double-stranded DNA viruses belonging to the proposed order "Megavirales." These viruses have a circular genome of ∼370 kbp, coding hundreds of genes. Over a half of their genes are associated with AT-rich putative promoter motifs, which have been demonstrated to be important for gene regulation. However, the transcriptional profile of Marseilleviruses is currently unknown. Here we used RNA sequencing technology to get a general transcriptional profile of Marseilleviruses. Eight million 75-bp-long nucleotide sequences were robustly mapped to all 457 genes initially predicted for Marseillevirus isolate T19, the prototype strain of the family, and we were able to assemble 359 viral contigs using a genome-guided approach with stringent parameters. These reads were differentially mapped to the genes according to the replicative cycle time point from which they were obtained. Cluster analysis indicated the existence of three main temporal categories of gene expression, early, intermediate and late, which were validated by quantitative reverse transcription polymerase chain reaction assays targeting several genes. Genes belonging to different functional groups exhibited distinct expression levels throughout the infection cycle. We observed that the previously predicted promoter motif, AAATATTT, as well as new predicted motifs, were not specifically related to any of the temporal or functional classes of genes, suggesting that other components are involved in temporally regulating virus transcription. Moreover, the host transcription machinery is heavily altered, and many genes are down regulated, including those related to translation process. This study provides an overview of the transcriptional landscape of Marseilleviruses.
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