Evidence map›Paper›PMID 32389302›Full record

ArticleFungal biology2020

Evaluating the circadian rhythm and response to glucose addition in dispersed growth cultures of Neurospora crassa.

Christina M Kelliher, Jennifer J Loros, Jay C Dunlap

Open access · greenAbstract read
In one paragraph

Article in Fungal biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
0.9field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 14 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Circadian rhythms: pervasive, and often times evasive.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025
    Review
  5. Article
  6. Article
  7. PRD-2 mediates clock-regulated perinuclear localization of clock gene RNAs within the circadian cycle ofProceedings of the National Academy of Sciences of the United States of America · 2022
    Article
  8. Article
  9. Article
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Christina M KelliherDepartment of Molecular & Systems Biology, Geisel School of Medicine at Dartmouth, Hanover, NH, USA.
Jennifer J LorosDepartment of Biochemistry & Cell Biology, Geisel School of Medicine at Dartmouth, Hanover, NH, USA.
Jay C DunlapDepartment of Molecular & Systems Biology, Geisel School of Medicine at Dartmouth, Hanover, NH, USA. Electronic address: jay.c.dunlap@dartmouth.edu.
Dartmouth College · US

Funding

Genetic and Molecular Dissection of the Neurospora ClockR35GM118021 · NIGMS · DARTMOUTH COLLEGE · PI Jay C. Dunlap · 2016 to 2026
$7.3M
Identification and Analysis of Circadian Clock-Controlled GenesR35GM118022 · NIGMS · DARTMOUTH COLLEGE · PI LOROS, JENNIFER J. · 2016 to 2021
$3.7M
Multiscale Modeling of Circadian RhythmsU01EB022546 · NIBIB · BATTELLE PACIFIC NORTHWEST LABORATORIES · PI CANNON, WILLIAM R, DUNLAP, JAY C. · 2016 to 2019
$2.3M
Genetic and Molecular Dissection of Regulatory Mechanisms Underlying Temperature and Nutritional Compensation of the Circadian Clock in Neurospora crassaF32GM128252 · NIGMS · DARTMOUTH COLLEGE · PI KELLIHER, CHRISTINA · 2018 to 2022
$210k
NIBIB NIH HHS U01 EB022546NIGMS NIH HHS F32 GM128252NIGMS NIH HHS R35 GM118021NIGMS NIH HHS R35 GM118022
6 · The paper itself

Abstract

Work on the filamentous fungus Neurospora crassa has contributed to or pioneered many aspects of research on circadian clock mechanism, a process that is functionally conserved across eukaryotes. Biochemical assays of the fungal circadian clock typically involve growth in liquid medium where Neurospora forms a spherical ball of submerged mycelium. Here, we revive a method for dispersed growth of Neurospora in batch culture using polyacrylic acid as an additive to the medium. We demonstrate that dispersed growth cultures utilize more carbon than mycelial balls, but nonetheless retain a functional circadian clock. This culturing method is suited for use in circadian experiments where uniform exposure to nutrients and/or increased biomass is required.

Indexed as

Circadian RhythmGlucoseNeurospora crassaCulture TechniquesGlucoseCircadian clockGlucose utilizationLuciferase reporterPolyacrylic acid

Identifiers

PMID32389302
PMCPMC7217977
OpenAlexW2991223423

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.