Evidence map›Paper›PMID 32389049›Full record

ArticleThe Journal of international medical research2020

Cleavage of cyclic AMP-responsive element-binding protein H aggravates myocardial hypoxia reperfusion injury in a hepatocyte-myocardial cell co-culture system.

Zehao Jin, Ye Chen, Xiaochun Weng, Anwu Huang, Shuang Lin, Haiying Li

RetractedOpen access · goldAbstract readRetracted Publication
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of international medical research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 50% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

  • Retraction · 2024-01-13Concerns/Issues about Authorship/Affiliation · Concerns/Issues about Third Party Involvement · Investigation by Journal/Publisher · Paper Mill · Unreliable Results and/or Conclusions ·
  • Retracted
5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Zehao JinDepartment of Cardiology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.
Ye ChenDepartment of Cardiology, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.
Xiaochun WengDepartment of Cardiology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.
Anwu HuangDepartment of Cardiology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.
Shuang LinDepartment of Cardiology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.
Haiying LiDepartment of Cardiology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.ORCID https://orcid.org/0000-0003-1942-1430
Wenzhou Medical University · CNShenzhen University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveThis study aimed to determine whether proinflammatory cytokines have an effect on myocardial cells (MCs) and hepatocytes during myocardial ischemia to induce cyclic AMP-responsive element-binding protein H (CREBH) cleavage, activate the acute phase response in the liver, and cause a superimposed injury in MCs.

methodsIn this study, a hepatocyte-MC transwell co-culture system was used to investigate the relationship between myocardial hypoxia/reperfusion injury and CREBH cleavage. MCs and hepatocytes of neonatal rats were obtained from the ventricles and livers of Sprague-Dawley rats, respectively. MCs were inoculated into the lower chamber of transwell chambers for 12 hours under hypoxia. Levels of the endoplasmic reticulum stress protein glucose-regulated protein 78 in MCs, CREBH in hepatocytes, inflammatory factor (tumor necrosis factor-α and interleukin-6) levels, and cell viability were evaluated. The effect of CREBH knockdown was also studied using a CREBH-specific short hairpin RNA (Ad-CREBHi).

resultsWe found that proinflammatory cytokines affect MCs and hepatocytes during myocardial ischemia to induce CREBH cleavage, activate the acute phase response in the liver, and cause superimposed injury in MCs.

conclusionsExpression of CREBH aggravates myocardial injury during myocardial ischemia.

Indexed as

Acute-Phase ReactionAnimalsAnimals, NewbornCell HypoxiaCells, CulturedCell SurvivalCoculture TechniquesCyclic AMP Response Element-Binding ProteinDisease Models, AnimalEndoplasmic Reticulum Chaperone BiPEndoplasmic Reticulum StressGene Knockdown TechniquesHeat-Shock ProteinsHepatocytesHumansInterleukin-6Creb3l3 protein, ratCyclic AMP Response Element-Binding ProteinEndoplasmic Reticulum Chaperone BiPGRP78 protein, ratHeat-Shock ProteinsIl6 protein, ratInterleukin-6RNA, Small InterferingTumor Necrosis Factor-alphaAcute phase responseco-culture systemcyclic AMP-responsive element-binding protein Hendoplasmic reticulum stresshypoxia/reoxygenationinflammatory cytokinemyocardial hypoxia–reperfusion injury

Identifiers

PMID32389049
PMCPMC7221173
OpenAlexW3021448076

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.