ArticleJournal of biomedical optics2020
Monitoring calcium-induced epidermal differentiation in vitro using multiphoton microscopy.
Article in Journal of biomedical optics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 6 citations in OpenAlex.
- Insights into metabolic changes during epidermal differentiation as revealed by multiphoton microscopy with fluorescence lifetime imaging.Scientific reports · 2025Article
- Combined flat-field and frequency filter approach to correcting artifacts of multichannel two-photon microscopy.Journal of biomedical optics · 2024Article
- Label-free photothermal optical coherence microscopy to locate desired regions of interest in multiphoton imaging of volumetric specimens.Scientific reports · 2023Article
- Contribution of autofluorescence from intracellular proteins in multiphoton fluorescence lifetime imaging.Scientific reports · 2022Article
- Compartmentalized primary cultures of dorsal root ganglion neurons to model peripheral pathophysiological conditions.Molecular painArticle
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
significanceResearch in tissue engineering and in vitro organ formation has recently intensified. To assess tissue morphology, the method of choice today is restricted primarily to histology. Thus novel tools are required to enable noninvasive, and preferably label-free, three-dimensional imaging that is more compatible with futuristic organ-on-a-chip models.
aimWe investigate the potential for using multiphoton microscopy (MPM) as a label-free in vitro approach to monitor calcium-induced epidermal differentiation. APPROACH: In vitro epidermis was cultured at the air-liquid interface in varying calcium concentrations. Morphology and tissue architecture were investigated using MPM based on visualizing cellular autofluorescence.
resultsDistinct morphologies corresponding to epidermal differentiation were observed. In addition, Ca2 + -induced effects could be distinguished based on the architectural differences in stratification in the tissue cultures.
conclusionsOur study shows that MPM based on cellular autofluorescence enables visualization of Ca2 + -induced differentiation in epidermal skin models in vitro. The technique has potential to be further adapted as a noninvasive, label-free, and real-time tool to monitor tissue regeneration and organ formation in vitro.
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