ArticleBiotechnology and applied biochemistry2021
Betaine-assisted recombinase polymerase assay for rapid hepatitis B virus detection.
Article in Biotechnology and applied biochemistry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
- Advances in Rapid Nucleic Acid Diagnostics for Hepatitis B and C Viruses: A Comprehensive Review.Reviews in medical virology · 2026Review
- Enhancing Biosecurity in Mollusc Aquaculture: A Review of Current Isothermal Nucleic Acid Detection Methods.Animals : an open access journal from MDPI · 2025Review
- Recent Advances in Betaine: Extraction, Identification, and Bioactive Functions in Human Health.Food science & nutrition · 2025Review
- Article
- Chinese medicine in the treatment of chronic hepatitis B: The mechanisms of signal pathway regulation.Heliyon · 2024Review
- Point-of-Care Testing for Hepatitis Viruses: A Growing Need.Life (Basel, Switzerland) · 2023Review
- Lateral Flow Assay for Hepatitis B Detection: A Review of Current and New Assays.Micromachines · 2023Review
- Manually-Operated, Slider Cassette for Multiplexed Molecular Detection at the Point of Care.Sensors and actuators. B, Chemical · 2022Article
- Enhancement of a recombinase-aided amplification assay using betaine and pullulan.Infectious medicine · 2022Article
- Rapid Diagnostic Test for Hepatitis B Virus Viral Load Based on Recombinase Polymerase Amplification Combined with a Lateral Flow Read-Out.Diagnostics (Basel, Switzerland) · 2022Article
- Sensitive, Single-Pot, Two-Stage Assay for Hepatitis Viruses.Analytical chemistry · 2022Article
- Lateral flow assays (LFA) as an alternative medical diagnosis method for detection of virus species: The intertwine of nanotechnology with sensing strategies.Trends in analytical chemistry : TRAC · 2021Review
- Rapid Detection of Pathogens in Wound Exudate via Nucleic Acid Lateral Flow Immunoassay.Biosensors · 2021Article
- Simple and Feasible Detection of Hepatitis B VirusFrontiers in molecular biosciences · 2021Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Hepatitis B virus (HBV) is a worldwide epidemic pathogen that causes hepatitis B. On-site screening the HBV infection is of critical importance for preventing and diagnosing HBV infection. In this paper, a simple, visual, and rapid method for on-site detection of HBV-DNA has been developed. This method is based on betaine-assisted recombinase polymerase assay and followed with naked-eye detection via lateral flow assay (BRPA-LF). Result show that nonspecific amplification is prone to occur in recombinase polymerase amplification (RPA) if the assay was performed with serum sample without purification. This problem has been addressed by adding 0.8 M of betaine to the RPA reactions. It was demonstrated that BRPA-LF can detect 1,000 copies of HBV-DNA in 50 μL mixture, and achieved 90% sensitivity and 100% specificity for serum sample detection. These results demonstrated that BRPA-LF can resist serum interference and has great potential for on-site screening of HBV infection.
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Registered trials
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