ArticleDaru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences2020
Comparison of diclofenac with apigenin-glycosides rich Clinacanthus nutans extract for amending inflammation and catabolic protease regulations in osteoporotic-osteoarthritis rat model.
Article in Daru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 20 citations in OpenAlex.
- Estrogen deficiency as a common driver and integrative mechanism in osteoporosis‑osteoarthritis comorbidity (Review).Molecular medicine reports · 2026Review
- Bioactive Compounds from Propolis on Bone Homeostasis: A Narrative Review.Antioxidants (Basel, Switzerland) · 2025Review
- Comparison of passive and active motion: effect of myokine irisin on cartilage in knee osteoarthritis rats.Frontiers in physiology · 2025Article
- The Protective Activity of Apigenin Against Bone and Cartilage Diseases.Clinical interventions in aging · 2025Review
- Pharmacology and mechanisms of apigenin in preventing osteoporosis.Frontiers in pharmacology · 2024Review
- Antiviral and Immunomodulatory Activities ofInternational journal of molecular sciences · 2023Review
- Apigenin Inhibits the Progression of Osteoarthritis by Mediating Macrophage Polarization.Molecules (Basel, Switzerland) · 2023Article
- Identification of kukoamine a as an anti-osteoporosis drug target using network pharmacology and experiment verification.Molecular medicine (Cambridge, Mass.) · 2023Article
- Natural Approach in Osteoarthritis Therapy.Recent advances in inflammation & allergy drug discovery · 2022Review
- Anti-Inflammatory Effects ofEvidence-based complementary and alternative medicine : eCAM · 2022Article
- Therapeutic Single Compounds for Osteoarthritis Treatment.Pharmaceuticals (Basel, Switzerland) · 2021Review
- A Brief Review of Drugs and Supplements Testing in Induced Osteoarthritis Murine Models: Methodologies and Findings.Current health sciences journalReview
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundOsteoporotic-osteoarthritis is an incapacitating musculoskeletal illness of the aged.
objectivesThe anti-inflammatory and anti-catabolic actions of Diclofenac were compared with apigenin-C-glycosides rich Clinacanthus nutans (CN) leaf extract in osteoporotic-osteoarthritis rats.
methodsFemale Sprague Dawley rats were randomized into five groups (n = 6). Four groups were bilateral ovariectomised for osteoporosis development, and osteoarthritis were induced by intra-articular injection of monosodium iodoacetate (MIA) into the right knee joints. The Sham group was sham-operated, received saline injection and deionized drinking water. The treatment groups were orally given 200 or 400 mg extract/kg body weight or 5 mg diclofenac /kg body weight daily for 28 days. Articular cartilage and bone changes were monitored by gross and histological structures, micro-CT analysis, serum protein biomarkers, and mRNA expressions for inflammation and catabolic protease genes.
resultsHPLC analysis confirmed that apigenin-C-glycosides (shaftoside, vitexin, and isovitexin) were the major compounds in the extract. The extract significantly and dose-dependently reduced cartilage erosion, bone loss, cartilage catabolic changes, serum osteoporotic-osteoarthritis biomarkers (procollagen-type-II-N-terminal-propeptide PIINP; procollagen-type-I-N-terminal-propeptide PINP; osteocalcin), inflammation (IL-1β) and mRNA expressions for nuclear-factor-kappa-beta NF-κβ, interleukin-1-beta IL-1β, cyclooxygenase-2; and matrix-metalloproteinase-13 MMP13 activities, in osteoporotic-osteoarthritis rats comparable to Diclofenac.
conclusionThis study demonstrates that apigenin-C-glycosides at 400 mg CN extract/kg (about 0.2 mg apigenin-equivalent/kg) is comparable to diclofenac in suppressing inflammation and catabolic proteases for osteoporotic-osteoarthritis prevention. Graphical abstract.
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