ArticleScientific reports2020
Genome-wide 5-hydroxymethylcytosine (5hmC) emerges at early stage of in vitro differentiation of a putative hepatocyte progenitor.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Magnesium treatment on methylation changes of transmembrane serine protease 2 (TMPRSS2).Nutrition (Burbank, Los Angeles County, Calif.) · 2021Trial
- Low Depth Epigenetic Mapping of Maturation Versus Retrodifferentiation in HepaRG Cells.Epigenomes · 2026Article
- Fundamentals of DNA methylation in development.Pediatric research · 2025Review
- Cell identity and 5-hydroxymethylcytosine.Epigenetics & chromatin · 2025Review
- 20(S)-Ginsenoside Rg3 Partially Induces Maturation of HepG2 Cells via the AMPK/HNF4A Pathway.Iranian journal of public health · 2025Article
- Elucidating epigenetic landscape of gastric premalignant lesions through genome-wide mapping of 5-hydroxymethylcytosines: A 12-year median follow-up study.Clinical and translational medicine · 2024Article
- Single-Molecule DNA Methylation Reveals Unique Epigenetic Identity Profiles of T Helper Cells.Journal of immunology (Baltimore, Md. : 1950) · 2024Article
- TET2 Promotes Keloid Hyperplasia by Regulating 5hmC Modification in the TGFβ Promoter Region.Clinical, cosmetic and investigational dermatology · 2023Article
- Dynamic 5-Hydroxymethylcytosine Change: Implication for Aging of Non-Human Primate Brain.Epigenomes · 2022Article
- Depressive disorder and antidepressants from an epigenetic point of view.World journal of psychiatry · 2022Review
- Epigenetics, DNA damage, and aging.The Journal of clinical investigation · 2022Review
- Genome-wide 5-hydroxymethylcytosine (5hmC) reassigned in Pten-depleted mESCs along neural differentiation.Frontiers in cell and developmental biology · 2022Article
- Laboratory methods to decipher epigenetic signatures: a comparative review.Cellular & molecular biology letters · 2021Review
- Cell-free DNA 5-hydroxymethylcytosine profiles of long non-coding RNA genes enable early detection and progression monitoring of human cancers.Clinical epigenetics · 2021Article
- Cas9-targeted nanopore sequencing reveals epigenetic heterogeneity afterMicrobial genomics · 2021Article
- Low biological fluctuation of mitochondrial CpG and non-CpG methylation at the single-molecule level.Scientific reports · 2021Article
- COVID-19 Is a Multi-Organ Aggressor: Epigenetic and Clinical Marks.Frontiers in immunology · 2021Review
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11 authors.
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Abstract
A basic question linked to differential patterns of gene expression is how cells reach different fates despite using the same DNA template. Since 5-hydroxymethylcytosine (5hmC) emerged as an intermediate metabolite in active DNA demethylation, there have been increasing efforts to elucidate its function as a stable modification of the genome, including a role in establishing such tissue-specific patterns of expression. Recently we described TET1-mediated enrichment of 5hmC on the promoter region of the master regulator of hepatocyte identity, HNF4A, which precedes differentiation of liver adult progenitor cells in vitro. Here, we studied the genome-wide distribution of 5hmC at early in vitro differentiation of human hepatocyte-like cells. We found a global increase in 5hmC as well as a drop in 5-methylcytosine after one week of in vitro differentiation from bipotent progenitors, at a time when the liver transcript program is already established. 5hmC was overall higher at the bodies of overexpressed genes. Furthermore, by modifying the metabolic environment, an adenosine derivative prevents 5hmC enrichment and impairs the acquisition of hepatic identity markers. These results suggest that 5hmC could be a marker of cell identity, as well as a useful biomarker in conditions associated with cell de-differentiation such as liver malignancies.
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