ArticleJournal of biosciences2020
A human cell polarity protein Lgl2 regulates influenza A virus nucleoprotein exportation from nucleus in MDCK cells.
Article in Journal of biosciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
5 citing papers in PubMed, 5 citations in OpenAlex.
- MiR-139-5p/ENAH Affects Progression of Hepatocellular Carcinoma Cells.Biochemical genetics · 2022Article
- Tight Junctions, the Key Factor in Virus-Related Disease.Pathogens (Basel, Switzerland) · 2022Review
- Influenza Virus Infections in Polarized Cells.Viruses · 2022Review
- The Hypothiocyanite and Amantadine Combination Treatment Prevents Lethal Influenza A Virus Infection in Mice.Frontiers in immunology · 2022Article
- Strength in Diversity: Nuclear Export of Viral RNAs.Viruses · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study, the regulatory effect of the overexpression of polarity protein Lgl2 on the nuclear export of influenza A virus nucleoprotein in infected cells was investigated. A stable Tet-Off inducible MDCK cell line expressing a fusion protein comprising Lgl2 and an enhanced yellow fluorescent protein were used. TCID50 analysis and neuraminidase activity analysis revealed that replication of influenza A virus was inhibited in Lgl2 overexpressing cells. By immunofluorescence microscopical observation at different time point post virus infection, a retention of NP in cellular nucleus was found in Lgl2 overexpressing cells. Compared with normal MDCK cells, change in claudin-1 distribution between cell contacts caused by Lgl2 overexpression impaired the barrier function of tight junction. These results suggest that changes in cell polarity induced by Lgl2 overexpressing will affect virus NP transportation.
Indexed as
Identifiers
32385218W3027005231What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.