Evidence map›Paper›PMID 32384924›Full record

ArticleDiagnostic pathology2020

MicroRNA-15a-5p serves as a potential biomarker and regulates the viability and apoptosis of hippocampus neuron in children with temporal lobe epilepsy.

Na Li, Jingmei Pan, Wei Liu, Yuanyuan Li, Feng Li, Min Liu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Diagnostic pathology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed, 3 pooled it
1.8field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

23 citing papers in PubMed, 3 syntheses or guidelines pooled it, 36 citations in OpenAlex.

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  11. MicroRNAs in chronic pediatric diseases (Review).Experimental and therapeutic medicine · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Na LiDepartment of Pediatric Medicine, Yidu Central Hospital of Weifang, Weifang, Shandong, 262500, P.R. China.
Jingmei PanDepartment of Pediatric Medicine, Yidu Central Hospital of Weifang, Weifang, Shandong, 262500, P.R. China.
Wei LiuDepartment of Pediatric Medicine, Yidu Central Hospital of Weifang, Weifang, Shandong, 262500, P.R. China.
Yuanyuan LiDepartment of Medical Image, Yidu Central Hospital of Weifang, Weifang, Shandong, 262500, P.R. China.
Feng LiDepartment of Emergency Surgery, Weifang People's Hospital, No. 151, Guangwen Street, Weifang, Shandong, 261000, P.R. China. lifeng12_12@163.com.
Min LiuDepartment of Medical Insurance, Weifang Maternal and Child Health Care Hospital, Weifang, Shandong, 261021, P.R. China. minliuc5236@163.com.
Yidu Central Hospital of Weifang · CNWeifang Maternity and Child Care Hospital · CNWeifang People's Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundTemporal lobe epilepsy (TLE) is the most common type of epilepsy, usually starting in childhood. The dysregulation of microRNAs (miRNAs) has been identified in neurological disorders. The current study investigated the expression level and clinical significance of miR-15a-5p in TLE children, and explored its function in regulating cell behaviors of hippocampal neurons.

methodsThe expression level of miR-15a-5p was examined in the serum of 63 TLE children. Primary hippocampal cells were cultured in magnesium-free medium to mimic TLE condition in children. The effect of miR-15a-5p on the viability and apoptosis of hippocampus neuron was assessed using MTT or flow cytometric apoptosis assay.

resultsTLE children had significantly low expression of miR-15a-5p. MiR-15a-5p was of great value for the diagnosis of TLE in children, with high specificity and sensitivity. The expression level of miR-15a-5p was decreased significantly in hippocampal cells treated in the magnesium-free medium. Overexpression of miR-15a-5p attenuated TLE-induced reduction for cell viability, and reversed the cell apoptosis induced by TLE.

conclusionsMiR-15a-5p is downregulated in children with TLE, and overexpression of miR-15a-5p promoted the viability and inhibited the apoptosis of hippocampal neuron. MiR-15a-5p may be a promising biomarker for the diagnosis of children TLE.

Indexed as

AnimalsApoptosisBiomarkersCell SurvivalChildEpilepsy, Temporal LobeFemaleHippocampusHumansMaleMicroRNAsNeuronsRatsRats, WistarBiomarkersMicroRNAsMIRN15 microRNA, humanApoptosisHippocampus neuronmiR-15a-5pTemporal lobe epilepsyViability

Identifiers

PMID32384924
PMCPMC7206675
OpenAlexW3023888585

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.