ArticleCells2020
Integrative Cluster Analysis of Whole Hearts Reveals Proliferative Cardiomyocytes in Adult Mice.
Article in Cells, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 30 citations in OpenAlex.
- Rbp-Jκ controls NK cell late maturation and migration via chromatin landscape remodeling.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Article
- Insights into Cardiomyocyte Regeneration from Screening and Transcriptomics Approaches.International journal of molecular sciences · 2026Review
- [European journal of nuclear medicine and molecular imaging · 2026Article
- Cyclin A2 induces cytokinesis in human adult cardiomyocytes and drives reprogramming in mice.NPJ Regenerative medicine · 2025Article
- Spatial multiomics of acute myocardial infarction reveals immune cell infiltration through the endocardium.Nature cardiovascular research · 2025Article
- The trabecular and compact myocardium of adult vertebrate ventricles are transcriptionally similar despite morphological differences.Annals of the New York Academy of Sciences · 2025Article
- A Current Perspective of Medical Informatics Developments for a Clinical Translation of (Non-coding)RNAs and Single-Cell Technologies.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Distinct mononuclear diploid cardiac subpopulation with minimal cell-cell communications persists in embryonic and adult mammalian heart.Frontiers of medicine · 2023Article
- Review
- New Approaches in Heart Research: Prevention Instead of Cardiomyoplasty?International journal of molecular sciences · 2023Review
- RNA-Binding Protein LIN28a Regulates New Myocyte Formation in the Heart Through Long Noncoding RNA-H19.Circulation · 2023Article
- Identification and epidemiological characterization of Type-2 diabetes sub-population using an unsupervised machine learning approach.Nutrition & diabetes · 2022Article
- Cellular Heterogeneity of the Heart.Frontiers in cardiovascular medicine · 2022Review
- Advances in application of single-cell RNA sequencing in cardiovascular research.Frontiers in cardiovascular medicine · 2022Review
- Automated annotation of rare-cell types from single-cell RNA-sequencing data through synthetic oversampling.BMC bioinformatics · 2021Article
- Spaceflight Modulates the Expression of Key Oxidative Stress and Cell Cycle Related Genes in Heart.International journal of molecular sciences · 2021Article
- Article
- Review
- Understanding the Adult Mammalian Heart at Single-Cell RNA-Seq Resolution.Frontiers in cell and developmental biology · 2021Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The recent development and broad application of sequencing techniques at the single-cell level is generating an unprecedented amount of data. The different techniques have their individual limits, but the datasets also offer unexpected possibilities when utilized collectively. Here, we applied snRNA-seq in whole adult murine hearts from an inbred (C57BL/6NRj) and an outbred (Fzt:DU) mouse strain to directly compare the data with the publicly available scRNA-seq data of the tabula muris project. Explicitly choosing a single-nucleus approach allowed us to pin down the typical heart tissue-specific technical bias, coming up with novel insights on the mammalian heart cell composition. For our integrated dataset, cardiomyocytes, fibroblasts, and endothelial cells constituted the three main cell populations accounting for about 75% of all cells. However, their numbers severely differed between the individual datasets, with cardiomyocyte proportions ranging from about 9% in the tabula muris data to around 23% for our BL6 data, representing the prime example for cell capture technique related bias when using a conventional single-cell approach for these large cells. Most strikingly in our comparison was the discovery of a minor population of cardiomyocytes characterized by proliferation markers that could not be identified by analyzing the datasets individually. It is now widely accepted that the heart has an, albeit very restricted, regenerative potential. However there is still an ongoing debate where new cardiomyocytes arise from. Our findings support the idea that the renewal of the cardiomyocyte pool is driven by cytokinesis of resident cardiomyocytes rather than differentiation of progenitor cells. We thus provide data that can contribute to an understanding of heart cell regeneration, which is a prerequisite for future applications to enhance the process of heart repair.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.