Evidence map›Paper›PMID 32383867›Full record

ArticleACS synthetic biology2020

Bottom-Up Construction of a Minimal System for Cellular Respiration and Energy Regeneration.

Olivier Biner, Justin G Fedor, Zhan Yin, Judy Hirst

Open access · greenAbstract read
In one paragraph

Article in ACS synthetic biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.

0numbers the graph read from it
0cells of the map it votes in
41citing papers in PubMed
3.3field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

41 citing papers in PubMed, 75 citations in OpenAlex.

  1. Functional Reconstitution of Mitochondrial Respiratory Supercomplexes.Journal of visualized experiments : JoVE · 2026
    Article
  2. Review
  3. Article
  4. Article
  5. Membrane-Associated Biomolecules for Synthetic Cell Signalling.Chembiochem : a European journal of chemical biology · 2026
    Review
  6. Improving cell-free metabolism through direct integration of artificial respiratory chains.Proceedings of the National Academy of Sciences of the United States of America · 2026
    Article
  7. Quinones operate as proton-collecting antennas in energy-transducing membranes.Proceedings of the National Academy of Sciences of the United States of America · 2026
    Article
  8. Article
  9. Global conformations ofScience advances · 2025
    Article
  10. Reconstituting alternative life using the test-bed of cell-free systems.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025
    Review
  11. Article
  12. Article
  13. Photoreceptor-Like Signal Transduction Between Polymer-Based Protocells.Advanced materials (Deerfield Beach, Fla.) · 2025
    Article
  14. Article
  15. Article
  16. Review
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Olivier BinerMRC Mitochondrial Biology Unit, University of Cambridge, The Keith Peters Building, Cambridge Biomedical Campus, Hills Road, Cambridge, CB2 0XY, United Kingdom.ORCID 0000-0001-6935-2475
Justin G FedorMRC Mitochondrial Biology Unit, University of Cambridge, The Keith Peters Building, Cambridge Biomedical Campus, Hills Road, Cambridge, CB2 0XY, United Kingdom.
Zhan YinMRC Mitochondrial Biology Unit, University of Cambridge, The Keith Peters Building, Cambridge Biomedical Campus, Hills Road, Cambridge, CB2 0XY, United Kingdom.
Judy HirstMRC Mitochondrial Biology Unit, University of Cambridge, The Keith Peters Building, Cambridge Biomedical Campus, Hills Road, Cambridge, CB2 0XY, United Kingdom.ORCID 0000-0001-8667-6797
University of Cambridge · GB

Funding

Medical Research Council MC_UU_00015/2Medical Research Council MC_UU_00015/7
6 · The paper itself

Abstract

Adenosine triphosphate (ATP), the cellular energy currency, is essential for life. The ability to provide a constant supply of ATP is therefore crucial for the construction of artificial cells in synthetic biology. Here, we describe the bottom-up assembly and characterization of a minimal respiratory system that uses NADH as a fuel to produce ATP from ADP and inorganic phosphate, and is thus capable of sustaining both upstream metabolic processes that rely on NAD

Indexed as

Energy MetabolismAdenosine TriphosphateAlternative OxidaseCell-Free SystemElectron Transport Complex ILiposomesMitochondriaMitochondrial ProteinsMitochondrial Proton-Translocating ATPasesNADOxidoreductasesPlant ProteinsSynthetic BiologyAdenosine TriphosphateAlternative OxidaseElectron Transport Complex IF1F0-ATP synthaseLiposomesMitochondrial ProteinsMitochondrial Proton-Translocating ATPasesNADOxidoreductasesPlant Proteinsalternative oxidaseartificial cellATP synthasecomplex Ico-reconstitutionrespiratory chainsynthetic biology

Identifiers

PMID32383867
PMCPMC7611821
OpenAlexW3021703815

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.