ArticleCommunications biology2020
Ultra-high throughput single-cell analysis of proteins and RNAs by split-pool synthesis.
Article in Communications biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed.
- The genetic signature of memory encoding along the human hippocampal axis.bioRxiv : the preprint server for biology · 2026Article
- Evaluating the practical aspects and performance of commercial single-cell RNA sequencing technologies.NAR genomics and bioinformatics · 2026Article
- An engineered U7 small nuclear RNA scaffold greatly increases ADAR-mediated programmable RNA base editing.Nature communications · 2025Article
- Article
- scRNA seq of an F1 cross of Marek's disease resistant and susceptible chickens identifies allele specific expression signatures enriched in transcription modulators.Scientific reports · 2025Article
- Revealing gene function with statistical inference at single-cell resolution.Nature reviews. Genetics · 2024Review
- Dasatinib overcomes glucocorticoid resistance in B-cell acute lymphoblastic leukemia.Nature communications · 2023Article
- Multi-omic single cell sequencing: Overview and opportunities for kidney disease therapeutic development.Frontiers in molecular biosciences · 2023Review
- Simultaneous Single-Cell Profiling of the Transcriptome and Accessible Chromatin Using SHARE-seq.Methods in molecular biology (Clifton, N.J.) · 2023Article
- High-Throughput Synthesis of Thin Films for the Discovery of Energy Materials: A Perspective.ACS materials Au · 2022Review
- Quantifying protein abundance on single cells using split-pool sequencing on DNA-barcoded antibodies for diagnostic applications.Scientific reports · 2022Article
- Article
Corrections and comments
- Erratum issued
Authors and funding
20 authors.
Funding
Abstract
Single-cell omics provide insight into cellular heterogeneity and function. Recent technological advances have accelerated single-cell analyses, but workflows remain expensive and complex. We present a method enabling simultaneous, ultra-high throughput single-cell barcoding of millions of cells for targeted analysis of proteins and RNAs. Quantum barcoding (QBC) avoids isolation of single cells by building cell-specific oligo barcodes dynamically within each cell. With minimal instrumentation (four 96-well plates and a multichannel pipette), cell-specific codes are added to each tagged molecule within cells through sequential rounds of classical split-pool synthesis. Here we show the utility of this technology in mouse and human model systems for as many as 50 antibodies to targeted proteins and, separately, >70 targeted RNA regions. We demonstrate that this method can be applied to multi-modal protein and RNA analyses. It can be scaled by expansion of the split-pool process and effectively renders sequencing instruments as versatile multi-parameter flow cytometers.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.