ArticleScientific reports2020
Raman spectroscopic signatures of carotenoids and polyenes enable label-free visualization of microbial distributions within pink biofilms.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 58 citations in OpenAlex.
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- Broadband electronic resonance coherent anti-Stokes/Stokes Raman scattering microscopy.Science advances · 2026Article
- Characterization of theInternational journal of molecular sciences · 2026Article
- The Next Frontier in Vibrational Imaging: Chemically Designed Raman Probes Pass the Spectral Barrier.JACS Au · 2026Review
- Versatile water-floated nanostructures for three-dimensional nanotransfer printing.Nature communications · 2026Article
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- Chemical Diversity of Carotenoids Derived from Aquatic Animals and their Therapeutic, Biomedical, and Natural Colorant Applications.Combinatorial chemistry & high throughput screening · 2026Review
- Recent advances in carotenoid absorption, distribution, and elimination.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2025Review
- Single-cell pigment analysis of phototrophic and phyllosphere bacteria using simultaneous detection of Raman and autofluorescence spectra.Applied and environmental microbiology · 2025Article
- Transcriptional heterogeneity of catabolic genes on the plasmid pCAR1 causes host-specific carbazole degradation.Applied and environmental microbiology · 2024Article
- Isomerization of carotenoids in photosynthesis and metabolic adaptation.Biophysical reviews · 2023Review
- Simultaneous Imaging and Characterization of Polyunsaturated Fatty Acids, Carotenoids, and Microcrystalline Guanine in SingleThe journal of physical chemistry. B · 2023Article
- Raman Spectroscopy of Carotenoid Compounds for Clinical Applications-A Review.Molecules (Basel, Switzerland) · 2022Review
- Nondestructive microbial discrimination using single-cell Raman spectra and random forest machine learning algorithm.STAR protocols · 2022Article
- Raman Spectroscopy for Chemical Biology Research.Journal of the American Chemical Society · 2022Review
- CarotegenicBiology · 2022Article
- Rapid In Situ Detection of THC and CBD inAnalytical chemistry · 2022Article
- Quantitative Raman Analysis of Carotenoid Protein Complexes in Aqueous Solution.Molecules (Basel, Switzerland) · 2022Article
- Resonance Raman analysis of intracellular vitamin BAnalytical science advances · 2022Article
- Diagnosing COVID-19 in human serum using Raman spectroscopy.Lasers in medical science · 2022Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pink biofilms are multispecies microbial communities that are commonly found in moist household environments. The development of this pink stain is problematic from an aesthetic point of view, but more importantly, it raises hygienic concerns because they may serve as a potential reservoir of opportunistic pathogens. Although there have been several studies of pink biofilms using molecular analysis and confocal laser scanning microscopy, little is known about the spatial distributions of constituent microorganisms within pink biofilms, a crucial factor associated with the characteristics of pink biofilms. Here we show that Raman spectroscopic signatures of intracellular carotenoids and polyenes enable us to visualize pigmented microorganisms within pink biofilms in a label-free manner. We measured space-resolved Raman spectra of a pink biofilm collected from a bathroom, which clearly show resonance Raman bands of carotenoids. Multivariate analysis of the Raman hyperspectral imaging data revealed the presence of typical carotenoids and structurally similar but different polyenes, whose spatial distributions within the pink biofilm were found to be mutually exclusive. Raman measurements on individual microbial cells isolated from the pink biofilm confirmed that these distributions probed by carotenoid/polyene Raman signatures are attributable to different pigmented microorganisms. The present results suggest that Raman microspectroscopy with a focus on microbial pigments such as carotenoids is a powerful nondestructive method for studying multispecies biofilms in various environments.
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