ArticlePain2020
Assigning transcriptomic class in the trigeminal ganglion using multiplex in situ hybridization and machine learning.
Article in Pain, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 44 citations in OpenAlex.
- Protocol for targeted peripheral adeno-associated viral vector transduction of the intradental neurons in mice.STAR protocols · 2026Article
- Substance P and somatostatin neurons limit facial itch by recruiting distinct nociceptive circuits in the brainstem.Communications biology · 2026Article
- Tooth Pulp Afferents and Transient Receptor Potential (TRP) Ion Channels as Key Regulators of Pulp Homeostasis, Inflammation, and Pain.International journal of molecular sciences · 2025Review
- Article
- Nerve Types and Classification of Neurons Innervating Oral Cancer.Clinical cancer research : an official journal of the American Association for Cancer Research · 2025Article
- Distinct Neural Mechanisms in the Propagation of Itch in the Face and Body.Research square · 2025Article
- Investigating Mechanically Activated Currents from Trigeminal Neurons of Nonhuman Primates.eNeuro · 2025Article
- Meningeal brain borders and migraine headache genesis.Trends in neurosciences · 2024Review
- Sensitization of meningeal afferents to locomotion-related meningeal deformations in a migraine model.eLife · 2024Article
- The anatomy, neurophysiology, and cellular mechanisms of intradental sensation.Frontiers in pain research (Lausanne, Switzerland) · 2024Review
- Sensitization of meningeal afferents to locomotion-related meningeal deformations in a migraine model.bioRxiv : the preprint server for biology · 2023Article
- Calretinin-expressing islet cells are a source of pre- and post-synaptic inhibition of non-peptidergic nociceptor input to the mouse spinal cord.Scientific reports · 2023Article
- Meningeal Mechanisms and the Migraine Connection.Annual review of neuroscience · 2023Review
- Calretinin-expressing islet cells: a source of pre- and post-synaptic inhibition of non-peptidergic nociceptor input to the mouse spinal cord.bioRxiv : the preprint server for biology · 2023Article
- Trigeminal afferents sense locomotion-related meningeal deformations.Cell reports · 2022Article
- Article
- Human and mouse trigeminal ganglia cell atlas implicates multiple cell types in migraine.Neuron · 2022Article
- Anatomical Analysis of Transient Potential Vanilloid Receptor 1 (Frontiers in molecular neuroscience · 2022Article
- CGRP and the Calcitonin Receptor are Co-Expressed in Mouse, Rat and Human Trigeminal Ganglia Neurons.Frontiers in physiology · 2022Article
- Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
abstractSingle cell sequencing has provided unprecedented information about the transcriptomic diversity of somatosensory systems. Here, we describe a simple and versatile in situ hybridization (ISH)-based approach for mapping this information back to the tissue. We illustrate the power of this approach by demonstrating that ISH localization with just 8 probes is sufficient to distinguish all major classes of neurons in sections of the trigeminal ganglion. To further simplify the approach and make transcriptomic class assignment and cell segmentation automatic, we developed a machine learning approach for analyzing images from multiprobe ISH experiments. We demonstrate the power of in situ class assignment by examining the expression patterns of voltage-gated sodium channels that play roles in distinct somatosensory processes and pain. Specifically, this analysis resolves intrinsic problems with single cell sequencing related to the sparseness of data leading to ambiguity about gene expression patterns. We also used the multiplex in situ approach to study the projection fields of the different neuronal classes. Our results demonstrate that the surface of the eye and meninges are targeted by broad arrays of neural classes despite their very different sensory properties but exhibit idiotypic patterns of innervation at a quantitative level. Very surprisingly, itch-related neurons extensively innervated the meninges, indicating that these transcriptomic cell classes are not simply labeled lines for triggering itch. Together, these results substantiate the importance of a sensory neuron's peripheral and central connections as well as its transcriptomic class in determining its role in sensation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.