ArticleScientific reports2020
A Penicillium rubens platform strain for secondary metabolite production.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 82 citations in OpenAlex.
- Article
- A penta-segmented chrysovirus from Penicillium rubens expands the genomic diversity of alphachrysoviruses.Archives of virology · 2026Article
- Genetic Dereplication of MultipleJournal of natural products · 2026Article
- Environmental Compatibility ofJournal of fungi (Basel, Switzerland) · 2025Article
- Regulation and induction of fungal secondary metabolites: a comprehensive review.Archives of microbiology · 2025Review
- Application of multiple genomic-editing technologies inSynthetic and systems biotechnology · 2025Article
- Synthetic Biology in Natural Product Biosynthesis.Chemical reviews · 2025Review
- Targeted discovery of polyketides with antioxidant activity through integrated omics and cocultivation strategies.Applied and environmental microbiology · 2024Article
- Construction of an expression platform for fungal secondary metabolite biosynthesis in Penicillium crustosum.Applied microbiology and biotechnology · 2024Article
- Heterologous Naringenin Production in the Filamentous FungusJournal of agricultural and food chemistry · 2023Article
- Investigating the Stirred Tank Bioreactor Co-Cultures of the Secondary Metabolite ProducersBiomolecules · 2023Article
- CRISPR/Cas9-Mediated Disruption of theJournal of fungi (Basel, Switzerland) · 2023Article
- Fungal BGCs for Production of Secondary Metabolites: Main Types, Central Roles in Strain Improvement, and Regulation According to the Piano Principle.International journal of molecular sciences · 2023Review
- An efficient Agrobacterium-mediated system based on the pyrG auxotrophic marker for recombinant expression in the filamentous fungus Penicillium rubens.Biotechnology letters · 2023Article
- Titer improvement of mycophenolic acid in the novel producer strain Penicillium arizonense and expression analysis of its biosynthetic genes.BMC microbiology · 2023Article
- Advances and Challenges in CRISPR/Cas-Based Fungal Genome Engineering for Secondary Metabolite Production: A Review.Journal of fungi (Basel, Switzerland) · 2023Review
- A genetic tool to express long fungal biosynthetic genes.Fungal biology and biotechnology · 2023Article
- Deciphering chemical logic of fungal natural product biosynthesis through heterologous expression and genome mining.Natural product reports · 2023Review
- A Versatile in Vivo DNA Assembly Toolbox for Fungal Strain Engineering.ACS synthetic biology · 2022Article
- Co-cultivation of filamentous microorganisms in the presence of aluminum oxide microparticles.Applied microbiology and biotechnology · 2022Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We present a Penicillium rubens strain with an industrial background in which the four highly expressed biosynthetic gene clusters (BGC) required to produce penicillin, roquefortine, chrysogine and fungisporin were removed. This resulted in a minimal secondary metabolite background. Amino acid pools under steady-state growth conditions showed reduced levels of methionine and increased intracellular aromatic amino acids. Expression profiling of remaining BGC core genes and untargeted mass spectrometry did not identify products from uncharacterized BGCs. This platform strain was repurposed for expression of the recently identified polyketide calbistrin gene cluster and achieved high yields of decumbenone A, B and C. The penicillin BGC could be restored through in vivo assembly with eight DNA segments with short overlaps. Our study paves the way for fast combinatorial assembly and expression of biosynthetic pathways in a fungal strain with low endogenous secondary metabolite burden.
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