Evidence map›Paper›PMID 32376840›Full record

ArticleScientific reports2020

Discovering the hidden messages within cell trajectories using a deep learning approach for in vitro evaluation of cancer drug treatments.

A Mencattini, D Di Giuseppe, M C Comes, P Casti, F Corsi, F R Bertani, L Ghibelli, L Businaro, C Di Natale, M C Parrini and 1 more

Open access · goldAbstract read
In one paragraph

Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed
7.2field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 63 citations in OpenAlex.

  1. Leveraging Microphysiological Systems to Facilitate Neutrophil-Based Cancer Immunotherapy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
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  13. Systematic data analysis pipeline for quantitative morphological cell phenotyping.Computational and structural biotechnology journal · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 2 countries.

A MencattiniDepartment of Electronic Engineering, University of Rome Tor Vergata, Rome, Italy.ORCID http://orcid.org/0000-0002-3753-0457
D Di GiuseppeDepartment of Electronic Engineering, University of Rome Tor Vergata, Rome, Italy.
M C ComesDepartment of Electronic Engineering, University of Rome Tor Vergata, Rome, Italy.
P CastiDepartment of Electronic Engineering, University of Rome Tor Vergata, Rome, Italy.
F CorsiDepartment of Chemical Science and Technologies, University of Rome Tor Vergata, Rome, Italy.
F R BertaniInstitute for Photonics and Nanotechnology, Italian National Research Council, 00156, Rome, Italy.
L GhibelliDepartment of Biology, University of Rome Tor Vergata, Rome, Italy.
L BusinaroInstitute for Photonics and Nanotechnology, Italian National Research Council, 00156, Rome, Italy.
C Di NataleDepartment of Electronic Engineering, University of Rome Tor Vergata, Rome, Italy.
M C ParriniInstitute Curie, Centre de Recherche, Paris Sciences et Lettres Research University, 75005, Paris, France.ORCID http://orcid.org/0000-0002-7082-9792
E MartinelliDepartment of Electronic Engineering, University of Rome Tor Vergata, Rome, Italy. martinelli@ing.uniroma2.it.
University of Rome Tor Vergata · ITNational Research Council · ITInstitut Curie · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We describe a novel method to achieve a universal, massive, and fully automated analysis of cell motility behaviours, starting from time-lapse microscopy images. The approach was inspired by the recent successes in application of machine learning for style recognition in paintings and artistic style transfer. The originality of the method relies i) on the generation of atlas from the collection of single-cell trajectories in order to visually encode the multiple descriptors of cell motility, and ii) on the application of pre-trained Deep Learning Convolutional Neural Network architecture in order to extract relevant features to be used for classification tasks from this visual atlas. Validation tests were conducted on two different cell motility scenarios: 1) a 3D biomimetic gels of immune cells, co-cultured with breast cancer cells in organ-on-chip devices, upon treatment with an immunotherapy drug; 2) Petri dishes of clustered prostate cancer cells, upon treatment with a chemotherapy drug. For each scenario, single-cell trajectories are very accurately classified according to the presence or not of the drugs. This original approach demonstrates the existence of universal features in cell motility (a so called "motility style") which are identified by the DL approach in the rationale of discovering the unknown message in cell trajectories.

Indexed as

Computational BiologyDrug Screening Assays, AntitumorMachine LearningAlgorithmsAntineoplastic AgentsBioengineeringCell TrackingHumansMolecular ImagingReproducibility of ResultsTime-Lapse ImagingAntineoplastic Agents

Identifiers

PMID32376840
PMCPMC7203117
OpenAlexW3022107493

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.