ArticleMolecular plant2020
Hetero-trans-β-Glucanase Produces Cellulose-Xyloglucan Covalent Bonds in the Cell Walls of Structural Plant Tissues and Is Stimulated by Expansin.
Article in Molecular plant, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 46 citations in OpenAlex.
- Genetic regulation of stem elongation and thickening in ornamental plants: a review.Horticulture research · 2026Article
- Fine-tuning xylan side chains for enhanced production of high-yield sugars, fine-shaped nanocrystals, and stable Pickering emulsions in Arabidopsis thaliana.Biotechnology for biofuels and bioproducts · 2026Article
- PtrVINV2 is dispensable for cellulose synthesis but essential for salt tolerance in Populus trichocarpa Torr. and Gray.Plant biotechnology journal · 2025Article
- Endophytic and Rhizospheric Microorganisms: An Alternative for Sustainable, Organic, and Regenerative Bioinput Formulations for Modern Agriculture.Microorganisms · 2025Review
- Structure and growth of plant cell walls.Nature reviews. Molecular cell biology · 2024Review
- Towards an understanding of the enzymatic degradation of complex plant mannan structures.World journal of microbiology & biotechnology · 2023Review
- Single-molecular insights into the breakpoint of cellulose nanofibers assembly during saccharification.Nature communications · 2023Article
- Shank-localized cell wall growth contributes to Arabidopsis root hair elongation.Nature plants · 2022Article
- Definition of the Acceptor Substrate Binding Specificity in Plant Xyloglucan Endotransglycosylases Using Computational Chemistry.International journal of molecular sciences · 2022Article
- Building an extensible cell wall.Plant physiology · 2022Article
- Molecular mechanisms of seed dormancy release by gas plasma-activated water technology.Journal of experimental botany · 2022Article
- Emerging Roles of β-Glucanases in Plant Development and Adaptative Responses.Plants (Basel, Switzerland) · 2022Review
- Expansin-mediated developmental and adaptive responses: A matter of cell wall biomechanics?Quantitative plant biology · 2022Review
- Ride to cell wall: Arabidopsis XTH11, XTH29 and XTH33 exhibit different secretion pathways and responses to heat and drought stress.The Plant journal : for cell and molecular biology · 2021Article
- Defining natural factors that stimulate and inhibit cellulose:xyloglucan hetero-transglucosylation.The Plant journal : for cell and molecular biology · 2021Article
- Cutin:cutin-acid endo-transacylase (CCT), a cuticle-remodelling enzyme activity in the plant epidermis.The Biochemical journal · 2021Article
- Plant Xyloglucan Xyloglucosyl Transferases and the Cell Wall Structure: Subtle but Significant.Molecules (Basel, Switzerland) · 2020Review
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Authors and funding
9 authors at 3 institutions in 3 countries.
Funding
Abstract
Current cell-wall models assume no covalent bonding between cellulose and hemicelluloses such as xyloglucan or mixed-linkage β-d-glucan (MLG). However, Equisetum hetero-trans-β-glucanase (HTG) grafts cellulose onto xyloglucan oligosaccharides (XGOs) - and, we now show, xyloglucan polysaccharide - in vitro, thus exhibiting CXE (cellulose:xyloglucan endotransglucosylase) activity. In addition, HTG also catalyzes MLG-to-XGO bonding (MXE activity). In this study, we explored the CXE action of HTG in native plant cell walls and tested whether expansin exposes cellulose to HTG by disrupting hydrogen bonds. To quantify and visualize CXE and MXE action, we assayed the sequential release of HTG products from cell walls pre-labeled with substrate mimics. We demonstrated covalent cellulose-xyloglucan bonding in plant cell walls and showed that CXE and MXE action was up to 15% and 60% of total transglucanase action, respectively, and peaked in aging, strengthening tissues: CXE in xylem and cells bordering intercellular canals and MXE in sclerenchyma. Recombinant bacterial expansin (EXLX1) strongly augmented CXE activity in vitro. CXE and MXE action in living Equisetum structural tissues potentially strengthens stems, while expansin might augment the HTG-catalyzed CXE reaction, thereby allowing efficient CXE action in muro. Our methods will enable surveys for comparable reactions throughout the plant kingdom. Furthermore, engineering similar hetero-polymer formation into angiosperm crop plants may improve certain agronomic traits such as lodging tolerance.
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