Evidence map›Paper›PMID 32375383›Full record

ArticleViruses2020

Merkel Cell Polyomavirus (MCPyV) in the Context of Immunosuppression: Genetic Analysis of Noncoding Control Region (NCCR) Variability among a HIV-1-Positive Population.

Carla Prezioso, Francisco Obregon, Donatella Ambroselli, Sara Petrolo, Paola Checconi, Donatella Maria Rodio, Luigi Coppola, Angelo Nardi, Corrado de Vito, Loredana Sarmati and 4 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Viruses, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
0.6field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 17 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 4 institutions in 1 country.

Carla PreziosoIRCSS San Raffaele Pisana, Microbiology of Chronic Neuro-Degenerative Pathologies, 00166 Rome, Italy.
Francisco ObregonDepartment of Public Health and Infectious Diseases, "Sapienza" University, 00185 Rome, Italy.
Donatella AmbroselliDepartment of Public Health and Infectious Diseases, "Sapienza" University, 00185 Rome, Italy.
Sara PetroloDepartment of Public Health and Infectious Diseases, "Sapienza" University, 00185 Rome, Italy.
Paola ChecconiIRCCS San Raffaele Pisana, Department of Human Sciences and Promotion of the Quality of Life, San Raffaele Roma Open University, 00166 Rome, Italy.
Donatella Maria RodioDepartment of Public Health and Infectious Diseases, "Sapienza" University, 00185 Rome, Italy.
Luigi CoppolaInfectious Diseases Clinic, Policlinic Tor Vergata, 00133 Rome, Italy.
Angelo NardiDepartment of Public Health and Infectious Diseases, "Sapienza" University, 00185 Rome, Italy.
Corrado de VitoDepartment of Public Health and Infectious Diseases, "Sapienza" University, 00185 Rome, Italy.
Loredana SarmatiInfectious Diseases Clinic, Policlinic Tor Vergata, 00133 Rome, Italy.ORCID 0000-0003-1452-0333
Massimo AndreoniInfectious Diseases Clinic, Policlinic Tor Vergata, 00133 Rome, Italy.
Anna Teresa PalamaraIRCSS San Raffaele Pisana, Microbiology of Chronic Neuro-Degenerative Pathologies, 00166 Rome, Italy.
Marco CiottiLaboratory of Clinical Microbiology and Virology, Polyclinic Tor Vergata Foundation, 00133 Rome, Italy.ORCID 0000-0002-9943-9130
Valeria PietropaoloDepartment of Public Health and Infectious Diseases, "Sapienza" University, 00185 Rome, Italy.ORCID 0000-0001-5723-8886
IRCCS Ospedale San Raffaele · ITPoliclinico Tor Vergata · ITUniversity of Rome Tor Vergata · ITIstituti di Ricovero e Cura a Carattere Scientifico · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundSince limited data are available about the prevalence of Merkel cell polyomavirus (MCPyV) and the genetic variability of its noncoding control region (NCCR) in the context of immunosuppression, this study aimed to investigate the distribution of MCPyV in anatomical sites other than the skin and the behavior of NCCR among an HIV-1-positive population.

methodsUrine, plasma, and rectal swabs specimens from a cohort of 66 HIV-1-positive patients were collected and subjected to quantitative real-time polymerase chain reaction (qPCR) for MCPyV DNA detection. MCPyV-positive samples were amplified by nested PCR targeting the NCCR, and NCCRs alignment was carried out to evaluate the occurrence of mutations and to identify putative binding sites for cellular factors.

resultsMCPyV DNA was detected in 10/66 urine, in 7/66 plasma, and in 23/66 rectal samples, with a median value of 5 × 10

conclusionsSequencing analysis revealed the presence of numerous mutations in the NCCR, including insertions and deletions. Whether these mutations may have an impact on the pathogenic features of the virus remains to be determined. qPCR measured on average a low viral load in the specimens analyzed, with the exception of those with the GTTGA insertion.

Indexed as

AdultAgedBase SequenceCohort StudiesCoinfectionCross-Sectional StudiesDNA, ViralFemaleHIV-1HIV InfectionsHumansImmunosuppression TherapyMaleMerkel cell polyomavirusMiddle AgedPolyomavirus InfectionsDNA, ViralRNA, UntranslatedGTT and GTTGA insertionsHIV-1-positive populationMerkel cell polyomavirusnoncoding control regionputative binding sites

Identifiers

PMID32375383
PMCPMC7291121
OpenAlexW3022247965

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.