ArticleCell reports2020
Variation of Human Neural Stem Cells Generating Organizer States In Vitro before Committing to Cortical Excitatory or Inhibitory Neuronal Fates.
Open access · goldAbstract read
In one paragraph
Article in Cell reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
2.7field-weighted citation impact, top 10% of its field
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
- Developmental signaling reveals functionally enriched human-specific gene regulation in telencephalic progenitors.bioRxiv : the preprint server for biology · 2026Article
- Clinical, cellular, and genomic consequences of a population-enrichedResearch square · 2026Article
- Human cortical organoids recapitulate inter-individual variability in infant brain-growth trajectories.Cell stem cell · 2026Article
- Radiofrequency regulates the BET-mediated pathways in radial glia differentiation in human cortical development.Cell reports · 2025Article
- Early developmental origins of cortical disorders modeled in human neural stem cells.Nature communications · 2025Article
- Specification of human brain regions with orthogonal gradients of WNT and SHH in organoids reveals patterning variations across cell lines.Cell stem cell · 2025Article
- Early cell cycle genes in cortical organoid progenitors predict interindividual variability in infant brain growth trajectories.bioRxiv : the preprint server for biology · 2025Article
- Individual variation in the emergence of anterior-to-posterior neural fates from human pluripotent stem cells.Stem cell reports · 2024Article
- Recapitulation of Perturbed Striatal Gene Expression Dynamics of Donors' Brains With Ventral Forebrain Organoids Derived From the Same Individuals With Schizophrenia.The American journal of psychiatry · 2024Article
- Gene expression signatures in blood from a West African sepsis cohort define host response phenotypes.Nature communications · 2024Article
- ERK signaling expands mammalian cortical radial glial cells and extends the neurogenic period.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- BMP7 expression in mammalian cortical radial glial cells increases the length of the neurogenic period.Protein & cell · 2024Article
- Molecular programs of regional specification and neural stem cell fate progression in macaque telencephalon.Science (New York, N.Y.) · 2023Article
- Modeling idiopathic autism in forebrain organoids reveals an imbalance of excitatory cortical neuron subtypes during early neurogenesis.Nature neuroscience · 2023Article
- Directional induction of neural stem cells, a new therapy for neurodegenerative diseases and ischemic stroke.Cell death discovery · 2023Review
- Coordinating cerebral cortical construction and connectivity: Unifying influence of radial progenitors.Neuron · 2022Review
- Engineering brain assembloids to interrogate human neural circuits.Nature protocols · 2022Review
- Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
23 authors at 7 institutions in 1 country.
Nicola MicaliLieber Institute for Brain Development, 855 North Wolfe St., Baltimore, MD 21205, USA; Department of Neuroscience, Yale School of Medicine, New Haven, CT 06520, USA. Electronic address: nicola.micali@yale.edu.
Suel-Kee KimLieber Institute for Brain Development, 855 North Wolfe St., Baltimore, MD 21205, USA; Department of Neuroscience, Yale School of Medicine, New Haven, CT 06520, USA.
Marcelo Diaz-BustamanteLieber Institute for Brain Development, 855 North Wolfe St., Baltimore, MD 21205, USA.
Genevieve Stein-O'BrienLieber Institute for Brain Development, 855 North Wolfe St., Baltimore, MD 21205, USA; McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA.
Seungmae SeoLieber Institute for Brain Development, 855 North Wolfe St., Baltimore, MD 21205, USA.
Joo-Heon ShinLieber Institute for Brain Development, 855 North Wolfe St., Baltimore, MD 21205, USA.
Brian G RashDepartment of Neuroscience, Yale School of Medicine, New Haven, CT 06520, USA.
Shaojie MaDepartments of Comparative Medicine, Genetics, and Psychiatry, Yale School of Medicine, New Haven, CT 06520, USA.
Yanhong WangLieber Institute for Brain Development, 855 North Wolfe St., Baltimore, MD 21205, USA.
Nicolas A OlivaresLieber Institute for Brain Development, 855 North Wolfe St., Baltimore, MD 21205, USA.
Jon I ArellanoDepartment of Neuroscience, Yale School of Medicine, New Haven, CT 06520, USA.
Kristen R MaynardLieber Institute for Brain Development, 855 North Wolfe St., Baltimore, MD 21205, USA.
Elana J FertigDepartment of Oncology, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA; Department of Biomedical Engineering, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA; Department of Applied Mathematics and Statistics, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA.
Alan J CrossAstraZeneca Neuroscience, IMED Biotech Unit, R&D, Boston, MA 024515, USA.
Roland W BürliAstraZeneca Neuroscience, IMED Biotech Unit, R&D, Boston, MA 024515, USA.
Nicholas J BrandonAstraZeneca Neuroscience, IMED Biotech Unit, R&D, Boston, MA 024515, USA.
Daniel R WeinbergerLieber Institute for Brain Development, 855 North Wolfe St., Baltimore, MD 21205, USA; McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA; Department of Psychiatry, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA; Department of Neuroscience, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA.
Joshua G ChenowethLieber Institute for Brain Development, 855 North Wolfe St., Baltimore, MD 21205, USA.
Daniel J HoeppnerLieber Institute for Brain Development, 855 North Wolfe St., Baltimore, MD 21205, USA; Astellas Research Institute of America, 3565 General Atomics Ct., Ste. 200, San Diego, CA 92121, USA.
Nenad SestanDepartment of Neuroscience, Yale School of Medicine, New Haven, CT 06520, USA; Departments of Comparative Medicine, Genetics, and Psychiatry, Yale School of Medicine, New Haven, CT 06520, USA; Kavli Institute for Neuroscience, Yale School of Medicine, New Haven, CT 06520, USA.
Pasko RakicDepartment of Neuroscience, Yale School of Medicine, New Haven, CT 06520, USA; Kavli Institute for Neuroscience, Yale School of Medicine, New Haven, CT 06520, USA. Electronic address: pasko.rakic@yale.edu.
Carlo ColantuoniLieber Institute for Brain Development, 855 North Wolfe St., Baltimore, MD 21205, USA; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA; Department of Neuroscience, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA; Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA. Electronic address: ccolantu@jhmi.edu.
Ronald D McKayLieber Institute for Brain Development, 855 North Wolfe St., Baltimore, MD 21205, USA. Electronic address: ronald.mckay@libd.org.
Lieber Institute for Brain Development · USYale University · USAstraZeneca (United States) · USJohns Hopkins University · USGeneral Atomics (United States) · USJohns Hopkins Hospital · USJohns Hopkins Medicine · US
Funding
Translational Research Central ServicesP30CA006973 · NCI · JOHNS HOPKINS UNIVERSITY · PI Rosalie Wollett · 1985 to 2026
$208.6MOrigin of Cortical Species-specific Distinctions.R01DA023999 · NIDA · YALE UNIVERSITY · PI RAKIC, PASKO · 2008 to 2019
$7.9M Functional Genomics of Human Brain DevelopmentP50MH106934 · NIMH · YALE UNIVERSITY · PI SESTAN, NENAD · 2014 to 2019
$7.9MPRENATAL DEVELOPMENT OF THE VISUAL SYSTEMR01EY002593 · NEI · YALE UNIVERSITY · PI ARELLANO, JUAN I, RAKIC, PASKO · 1985 to 2018
$6.5M1/2 Cell Type and Region-Specific Regulatory Networks in Human Brain Development and DisordersU01MH116488 · NIMH · YALE UNIVERSITY · PI SESTAN, NENAD · 2018 to 2022
$6.5MOrigin of Cortical Species-specific DistinctionsR37DA023999 · NIDA · YALE UNIVERSITY · PI PASKO RAKIC · 2020 to 2026
$5.6MYale Macaque Brain ResourceR01MH113257 · NIMH · YALE UNIVERSITY · PI Alvaro Duque · 2017 to 2026
$4.3M2/2 Somatic mosaicism and autism spectrum disorderU01MH106874 · NIMH · YALE UNIVERSITY · PI SESTAN, NENAD · 2015 to 2019
$4.0M1/3 Integrative Genomic Analysis of Human Brain Development and AutismR01MH110926 · NIMH · YALE UNIVERSITY · PI SESTAN, NENAD · 2016 to 2019
$3.2MIntegrating bioinformatics into multiscale models for hepatocellular carcinomaU01CA212007 · NCI · JOHNS HOPKINS UNIVERSITY · PI EWALD, ANDREW JOSEF, FERTIG, ELANA · 2018 to 2022
$3.2MDynamical Models of Cetuximab Resistance in HNSCC Based on Serial Genomics DataR01CA177669 · NCI · JOHNS HOPKINS UNIVERSITY · PI FERTIG, ELANA · 2014 to 2019
$1.8M3/3 Multidimensional investigation of the etiology of autism spectrum disorderR01MH109904 · NIMH · YALE UNIVERSITY · PI SESTAN, NENAD · 2016 to 2018
$816kNCI NIH HHS P30 CA006973NCI NIH HHS R01 CA177669NCI NIH HHS U01 CA212007NEI NIH HHS R01 EY002593NIDA NIH HHS R01 DA023999NIDA NIH HHS R37 DA023999NIMH NIH HHS P50 MH106934NIMH NIH HHS R01 MH109904NIMH NIH HHS R01 MH110926NIMH NIH HHS R01 MH113257NIMH NIH HHS U01 MH106874NIMH NIH HHS U01 MH116488
Abstract
Better understanding of the progression of neural stem cells (NSCs) in the developing cerebral cortex is important for modeling neurogenesis and defining the pathogenesis of neuropsychiatric disorders. Here, we use RNA sequencing, cell imaging, and lineage tracing of mouse and human in vitro NSCs and monkey brain sections to model the generation of cortical neuronal fates. We show that conserved signaling mechanisms regulate the acute transition from proliferative NSCs to committed glutamatergic excitatory neurons. As human telencephalic NSCs develop from pluripotency in vitro, they transition through organizer states that spatially pattern the cortex before generating glutamatergic precursor fates. NSCs derived from multiple human pluripotent lines vary in these early patterning states, leading differentially to dorsal or ventral telencephalic fates. This work furthers systematic analyses of the earliest patterning events that generate the major neuronal trajectories of the human telencephalon.
Indexed as
Cell DifferentiationCells, CulturedEmbryonic Stem CellsHumansInduced Pluripotent Stem CellsNeural Stem CellsNeurogenesisNeuronsSignal TransductionBMPbrain organizerEGFRFGF2glutamatergic neuronshuman iPSC variationinhibitory neuronsneural stem cellneural transcriptional dynamicsneurogenesisneuronal trajectory variationpatterning of the cortex
Identifiers
What OpenQuestion holds
Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390
Registered trials
None linked
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.