ArticleJournal of experimental botany2020
MS1, a direct target of MS188, regulates the expression of key sporophytic pollen coat protein genes in Arabidopsis.
Article in Journal of experimental botany, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
45 citing papers in PubMed, 84 citations in OpenAlex.
- Knockdown of Arabidopsis RUS4 impacts pollen coat formation by affecting flavonoid and lipid metabolism.Planta · 2026Article
- Pan-Genomic Dissection of GH1 β-Glucosidases inPlants (Basel, Switzerland) · 2026Article
- Molecular understanding of plant pollination, fertilization, seed development and protoplast regeneration.Science China. Life sciences · 2026Review
- Genome-wide characterization of the LBD gene family in Sapindus mukorossi and functional analysis of the role of SmuLBD26 in male sterility.Plant cell reports · 2026Article
- ICE1 plays a vital role in the development of pollen grains through regulating tapetum degradation and nutrient mobilization in Arabidopsis thaliana.Plant molecular biology · 2026Article
- Single-Cell Transcriptome Atlas and Dynamic Regulatory Mechanisms of Anther Development in Alfalfa (Medicago sativa L.).Plant biotechnology journal · 2025Article
- Characterization ofPlants (Basel, Switzerland) · 2025Article
- Insights into the Molecular Basis of Pollen Coat Development and Its Role in Male Sterility.International journal of molecular sciences · 2025Review
- Spatiotemporally transcriptomic analyses of floral buds reveal the high-resolution landscape of flower development and dormancy regulation in peach.Horticulture research · 2025Article
- Identity Transitions of Tapetum Phases: Insights into Vesicular Dynamics and in Mortem Support During Pollen Maturation.Plants (Basel, Switzerland) · 2025Review
- Microsporocytic ARF17 misexpression leads to an excess callose deposition and male sterility in Arabidopsis.Plant molecular biology · 2025Article
- Fine mapping and expression characteristics analysis of male-sterile gene BrRNR1 in Chinese cabbage (Brassica rapa L. ssp. pekinensis).BMC plant biology · 2025Article
- Genome-wide identification, transcript profiling and functional analyses of PCP gene family in Wucai (Brassica campestris).Scientific reports · 2024Article
- Identification of the new allele ptc1-2 and analysis of the regulatory role of PTC1 gene in rice anther development.BMC plant biology · 2024Article
- Analysis ofInternational journal of molecular sciences · 2024Article
- The BNB-GLID module regulates germline fate determination in Marchantia polymorpha.The Plant cell · 2024Article
- Environmental regulation of male fertility is mediated through Arabidopsis transcription factors bHLH89, 91, and 10.Journal of experimental botany · 2024Article
- Transcription Factors and Their Regulatory Roles in the Male Gametophyte Development of Flowering Plants.International journal of molecular sciences · 2024Review
- bHLH-regulated routes in anther development in rice and Arabidopsis.Genetics and molecular biology · 2024Article
- Single-Cell Transcriptome Atlas and Regulatory Dynamics in Developing Cotton Anthers.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
Abstract
Sporophytic pollen coat proteins (sPCPs) derived from the anther tapetum are deposited into pollen wall cavities and function in pollen-stigma interactions, pollen hydration, and environmental protection. In Arabidopsis, 13 highly abundant proteins have been identified in pollen coat, including seven major glycine-rich proteins GRP14, 16, 17, 18, 19, 20, and GRP-oleosin; two caleosin-related family proteins (AT1G23240 and AT1G23250); three lipase proteins EXL4, EXL5 and EXL6, and ATA27/BGLU20. Here, we show that GRP14, 17, 18, 19, and EXL4 and EXL6 fused with green fluorescent protein (GFP) are translated in the tapetum and then accumulate in the anther locule following tapetum degeneration. The expression of these sPCPs is dependent on two essential tapetum transcription factors, MALE STERILE188 (MS188) and MALE STERILITY 1 (MS1). The majority of sPCP genes are up-regulated within 30 h after MS1 induction and could be restored by MS1 expression driven by the MS188 promoter in ms188, indicating that MS1 is sufficient to activate their expression; however, additional MS1 downstream factors appear to be required for high-level sPCP expression. Our ChIP, in vivo transactivation assay, and EMSA data indicate that MS188 directly activates MS1. Together, these results reveal a regulatory cascade whereby outer pollen wall formation is regulated by MS188 followed by synthesis of sPCPs controlled by MS1.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.