Evidence map›Paper›PMID 32373934›Full record

ArticleBioscience reports2020

Genetic and expression variations of cell cycle pathway genes in brain tumor patients.

Anum Zehra Naqvi, Ishrat Mahjabeen, Saima Ameen, Malik Waqar Ahmed, Asad Ullah Khan, Zertashia Akram, Mahmood Akhtar Kayani

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioscience reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Anum Zehra NaqviCancer Genetics and Epigenetics Lab, Department of Biosciences COMSATS University, Islamabad, Pakistan.
Ishrat MahjabeenCancer Genetics and Epigenetics Lab, Department of Biosciences COMSATS University, Islamabad, Pakistan.
Saima AmeenCancer Genetics and Epigenetics Lab, Department of Biosciences COMSATS University, Islamabad, Pakistan.
Malik Waqar AhmedCancer Genetics and Epigenetics Lab, Department of Biosciences COMSATS University, Islamabad, Pakistan.
Asad Ullah KhanCancer Genetics and Epigenetics Lab, Department of Biosciences COMSATS University, Islamabad, Pakistan.
Zertashia AkramCancer Genetics and Epigenetics Lab, Department of Biosciences COMSATS University, Islamabad, Pakistan.
Mahmood Akhtar KayaniCancer Genetics and Epigenetics Lab, Department of Biosciences COMSATS University, Islamabad, Pakistan.
COMSATS University Islamabad · PK

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The present study was designed to determine the association between the genetic polymorphisms/expression variations of RB1 and CCND1 genes and brain tumor risk. For this purpose, 250 blood samples of brain tumor patients along with 250 controls (cohort I) and 96 brain tumor tissues (cohort II) with adjacent control section were collected. Mutation analysis of RB1 (rs137853294, rs121913300) and CCND1 (rs614367, rs498136) genes was performed using ARMS-PCR followed by sequencing, and expression analysis was performed using real-time PCR and immunohistochemistry. The results showed homozygous mutant genotype of RB1 gene polymorphism, rs121913300 (P=0.003) and CCND1 gene polymorphism rs614367 (P=0.01) were associated significantly with brain tumor risk. Moreover, significant down-regulation of RB1 (P=0.005) and up-regulation of CCND1 (P=0.0001) gene was observed in brain tumor sections vs controls. Spearman correlation showed significant negative correlation between RB1 vs proliferation marker, Ki-67 (r = -0.291*, P<0.05) in brain tumors. Expression levels of selected genes were also assessed at protein level using immunohistochemical analysis (IHC) and signification down-regulation of RB1 (P=0.0001) and up-regulation of CCND1 (P=0.0001) was observed in brain tumor compared with control sections. In conclusion, it is suggested that polymorphisms/expression variations of RB1 and CCND1 genes may be associated with increased risk of brain tumor.

Indexed as

Genetic Predisposition to DiseaseAdolescentAdultAgedAged, 80 and overBrainBrain NeoplasmsCell CycleChildCyclin D1Down-RegulationFemaleGene Expression Regulation, NeoplasticGene FrequencyHumansMaleCCND1 protein, humanCyclin D1RB1 protein, humanRetinoblastoma Binding ProteinsUbiquitin-Protein LigasesBrain tumorCCND1Expression analysisPolymorphismRB1

Identifiers

PMID32373934
PMCPMC7225413
OpenAlexW3021976410

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.