ArticleFrontiers in bioengineering and biotechnology2020
Cell-Laden Agarose-Collagen Composite Hydrogels for Mechanotransduction Studies.
Article in Frontiers in bioengineering and biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 73 citations in OpenAlex.
- Generation of Human Bioartificial Tissues Using Agarose-Derived Biomaterials.Materials (Basel, Switzerland) · 2026Review
- A tissue-intrinsic mechanism sensitizes HIV-1 particles for TLR-triggered innate immune responses.Nature communications · 2026Article
- TNF-α and IL-1β but not IL-18 counteract TGF-β1 mediated stimulation of collagen accumulation and increased extracellular matrix strength and stiffness.Scientific reports · 2026Article
- Design and characterisation of intervertebral disc mimicking phantoms for photoacoustic imaging.Photoacoustics · 2026Article
- In Vitro Experimental and Numerical Simulation Study on the Influence of Uniaxial Cyclic Compression on Cytoskeletal Structure.Bioengineering (Basel, Switzerland) · 2025Article
- Exploring Mechanotransduction and Inflammation in Human Cartilaginous Endplate Cells in Blended Collagen-Agarose Hydrogels Under Cyclic Compression.Gels (Basel, Switzerland) · 2025Article
- Regenerative strategies for intervertebral disc degeneration.Journal of orthopaedic translation · 2025Review
- Impact of Collagen on the Rheological and Transport Properties of Agarose Hydrogels.Gels (Basel, Switzerland) · 2025Article
- TNF induces catabolism in human cartilaginous endplate cells in 3D agarose culture under dynamic compression.Scientific reports · 2025Article
- A bioactive three-layered skin substitute based on ECM components effectively promotes skin wound healing and regeneration.Materials today. Bio · 2025Article
- A Bioinert Hydrogel Framework for Precision 3D Cell Cultures: Advancing Automated High-Content and High-Throughput Drug Screening.Small science · 2025Article
- Agarose Hydrogels for Bone Tissue Engineering, from Injectables to Bioprinting.Gels (Basel, Switzerland) · 2025Review
- Characterization of Composite Agarose-Collagen Hydrogels for Chondrocyte Culture.Annals of biomedical engineering · 2025Article
- Leveraging printability and biocompatibility in materials for printing implantable vessel scaffolds.Materials today. Bio · 2024Review
- Poly(Vinyl Alcohol) Drug and PVA-Drug-Surfactant Complex Organogel with Dimethyl Sulfoxide as a Drug Delivery System.Gels (Basel, Switzerland) · 2024Article
- Improved Composite Hydrogel for Bioengineered Tracheal Graft Demonstrates Effective Early Angiogenesis.Journal of clinical medicine · 2024Article
- Experimental Study on Compatibility of Human Bronchial Epithelial Cells in Collagen-Alginate Bioink for 3D Printing.Bioengineering (Basel, Switzerland) · 2024Article
- Agarose as a Tissue Mimic for the Porcine Heart, Kidney, and Liver: Measurements and a Springpot Model.Bioengineering (Basel, Switzerland) · 2024Article
- Precision Engineering of Chondrocyte Microenvironments: Investigating the Optimal Reaction Conditions for Type B Gelatin Methacrylate Hydrogel Matrix for TC28a2 Cells.Journal of functional biomaterials · 2024Article
- Nonspecific membrane-matrix interactions influence diffusivity of lipid vesicles in hydrogels.Biophysical journal · 2024Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The increasing investigation of cellular mechanotransduction mechanisms requires biomaterials combining biofunctionality and suitable mechanical properties. Agarose is a standard biomaterial for cartilage and intervertebral disc mechanobiology studies, but lacks adhesion motifs and the necessary cell-matrix interaction for mechanotransduction. Here, collagen type I was blended at two concentrations (2 and 4.5 mg/mL) with agarose 2% wt/vol. The composite hydrogels were characterized in terms of structural homogeneity, rheological properties and size stability. Nucleus pulposus (NP) cell viability, proliferation, morphology, gene expression, GAG production, adhesion and mechanotransduction ability were further tested. Blended hydrogels presented a homogenous network of the two polymers. While the addition of 4.5 mg/mL collagen significantly decreased the storage modulus and increased the loss modulus of the gels, blended gels containing 2 mg/mL collagen displayed similar mechanical properties to agarose. Hydrogel size was conserved over 21 days for all agarose-based gels. Embedded cells were viable (>80%) and presented reduced proliferation and a round morphology typical of NP cells
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.