Evidence map›Paper›PMID 32372170›Full record

ArticleMolecular biology reports2020

Replicative senescence in MSCWJ-1 human umbilical cord mesenchymal stem cells is marked by characteristic changes in motility, cytoskeletal organization, and RhoA localization.

Danila Bobkov, Anastasia Polyanskaya, Anastasia Musorina, Ekaterina Lomert, Sergey Shabelnikov, Galina Poljanskaya

Abstract read
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In one paragraph

Article in Molecular biology reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 13 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Danila BobkovCollection of Vertebrate Cell Cultures, Institute of Cytology, Russian Academy of Sciences, 4 Tikhoretsky Prospekt, St. Petersburg, Russia, 194064. bobkov@incras.ru.ORCID http://orcid.org/0000-0002-0358-9266
Anastasia PolyanskayaInstitute of Physics, Nanotechnology and Telecommunications, Peter the Great St. Petersburg Polytechnic University, 29 Polytechnicheskaya Ulitsa, St. Petersburg, Russia, 195251.ORCID http://orcid.org/0000-0002-8007-4872
Anastasia MusorinaCollection of Vertebrate Cell Cultures, Institute of Cytology, Russian Academy of Sciences, 4 Tikhoretsky Prospekt, St. Petersburg, Russia, 194064.ORCID http://orcid.org/0000-0003-3748-6545
Ekaterina LomertTumor Cell Molecular Genetics Group, Institute of Cytology, Russian Academy of Sciences, 4 Tikhoretsky Prospekt, St. Petersburg, Russia, 194064.ORCID http://orcid.org/0000-0003-3732-0553
Sergey ShabelnikovCell Technologies Center, Institute of Cytology, Russian Academy of Sciences, 4 Tikhoretsky Prospekt, St. Petersburg, Russia, 194064.ORCID http://orcid.org/0000-0002-5693-5310
Galina PoljanskayaCollection of Vertebrate Cell Cultures, Institute of Cytology, Russian Academy of Sciences, 4 Tikhoretsky Prospekt, St. Petersburg, Russia, 194064.ORCID http://orcid.org/0000-0002-6324-3603
Institute of Cytology · RUPeter the Great St. Petersburg Polytechnic University · RU

Funding

Russian Academy of Sciences АААА-А19-119020190093-9
6 · The paper itself

Abstract

Here, we document changes in cell motility and organization of the contractile apparatus of human umbilical cord Wharton's jelly mesenchymal stem cells (MSCWJ-1) in the process of replicative senescence. Colocalization dynamics of F-actin and actin-binding proteins (myosin-9, α-actinin-4, RhoA) were examined in the MSCWJ-1 cell line. The results show that nuclear-cytoplasmic redistribution of RhoA occurs during replicative senescence, with maximal RhoA/nucleus colocalization evident at passage 15. At that time point, decreases in colocalization, namely myosin-9/F-actin and α-actinin-4/F-actin, were seen and myosin-9 was found in cytosolic extracts in the assembly-incompetent form. Using an automated intravital confocal cytometry system and quantitative analysis of MSCWJ-1 movements, we found that changes in cytoskeletal organization correlate with cell motility characteristics over a time period from passages 9 to 38. The factors examined (cytoskeleton structure, cell motility) indicate that the process by which cells transition to replicative senescence is best represented as three stages. The first stage lasts from cell culture isolation to passage 15 and is characterized by: accumulation of actin-binding proteins in assembly-incompetent forms; nuclear RhoA accumulation; and an increase in movement tortuosity. The second stage extends from passages 15 to 28 and is characterized by: an increase in the structural integrity of the actin cytoskeleton; exit of RhoA and alpha-actinin-4 from the nucleus; and a decrease in path tortuosity. The third stage extends from passage 28 to 38 and is marked by: a plateau in actin cytoskeleton structural integrity; significant decreases in nuclear RhoA levels; and decreases in cell speed.

Indexed as

ActininActinsAgingCell LineCell MovementCell NucleusCellular SenescenceCytoskeletonFetal BloodHumansMesenchymal Stem CellsMyosinsrhoA GTP-Binding ProteinUmbilical CordActininActinsACTN4 protein, humanMyosinsrhoA GTP-Binding ProteinRHOA protein, humanActin cytoskeletonCell motilityCellular senescenceMesenchymal stem cellsRhoA

Identifiers

PMID32372170
OpenAlexW3022646848

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.