ArticleNature methods2020
Three-dimensional nanoscopy of whole cells and tissues with in situ point spread function retrieval.
Article in Nature methods, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
What it found
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Who cites it
44 citing papers in PubMed, 113 citations in OpenAlex.
- From stars to molecules: AI guided device-agnostic super-resolution imaging.Nature communications · 2026Article
- Digital-micromirror-device-based illumination strategies for background suppression in single-molecule localization microscopy.Biomedical optics express · 2026Article
- Aberration-aware 3D localization microscopy via self-supervised neural-physics learning.Nature communications · 2026Article
- Interferometric ultra-high resolution 3D imaging through brain sections.Nature communications · 2026Article
- Mirror-enhanced 4Pi-SMLM with one objective enables isotropic nanoscale imaging.Nature biotechnology · 2026Article
- Molecular-scale isotropic 3D super-resolution microscopy via interference localization.Nature methods · 2026Article
- Perspective: An outlook on fluorescence tracking.The Journal of chemical physics · 2025Article
- In-depth single molecule localization microscopy using adaptive optics and single objective light-sheet microscopy.Nature communications · 2025Article
- Model-free machine learning-based 3D single molecule localisation microscopy.Journal of microscopy · 2025Article
- Gradual labeling with fluorogenic probes: A general method for MINFLUX imaging and tracking.Science advances · 2025Article
- Simultaneous particle tracking, phase retrieval and point spread function reconstruction.bioRxiv : the preprint server for biology · 2025Article
- Enhancing ultra-high density single-molecule localization with deep spatiotemporal networks.Biomedical optics express · 2025Article
- Joint estimation of point spread function and molecule positions in SMLM informed from multiple planes.Biomedical optics express · 2025Article
- Single-molecule orientation-localization microscopy: Applications and approaches.Quarterly reviews of biophysics · 2024Review
- Calibration-free estimation of field dependent aberrations for single molecule localization microscopy across large fields of view.bioRxiv : the preprint server for biology · 2024Article
- Architecture-driven quantitative nanoscopy maps cytoskeleton remodeling.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Subcellular Drug Distribution: Exploring Organelle-Specific Characteristics for Enhanced Therapeutic Efficacy.Pharmaceutics · 2024Review
- Measurement precision bounds on aberrated single-molecule emission patterns.Optics express · 2024Article
- Nanoscale organization is changed in native, surface AMPARs by mouse brain region and tauopathy.bioRxiv : the preprint server for biology · 2024Article
- Universal inverse modeling of point spread functions for SMLM localization and microscope characterization.Nature methods · 2024Article
Corrections and comments
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Authors and funding
14 authors at 3 institutions in 1 country.
Funding
Abstract
Single-molecule localization microscopy is a powerful tool for visualizing subcellular structures, interactions and protein functions in biological research. However, inhomogeneous refractive indices inside cells and tissues distort the fluorescent signal emitted from single-molecule probes, which rapidly degrades resolution with increasing depth. We propose a method that enables the construction of an in situ 3D response of single emitters directly from single-molecule blinking datasets, and therefore allows their locations to be pinpointed with precision that achieves the Cramér-Rao lower bound and uncompromised fidelity. We demonstrate this method, named in situ PSF retrieval (INSPR), across a range of cellular and tissue architectures, from mitochondrial networks and nuclear pores in mammalian cells to amyloid-β plaques and dendrites in brain tissues and elastic fibers in developing cartilage of mice. This advancement expands the routine applicability of super-resolution microscopy from selected cellular targets near coverslips to intra- and extracellular targets deep inside tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.