ArticleBMC plant biology2020
Integration of sRNA, degradome, transcriptome analysis and functional investigation reveals gma-miR398c negatively regulates drought tolerance via GmCSDs and GmCCS in transgenic Arabidopsis and soybean.
Article in BMC plant biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 64 citations in OpenAlex.
- The Zma-miRNA319-ZmMYB74 Module Regulates Maize Resistance to Stalk Rot Disease by Modulating Lignin Deposition.Plant biotechnology journal · 2026Article
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- CRISPR/Cas9-Based Gene Editing in Soybean.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Article
- MicroRNAs-mediated heat stress regulations in plants: Mechanisms and targets.The plant genome · 2025Review
- Epigenomics and Non-Coding RNAs in Soybean Adaptation to Abiotic Stresses.International journal of molecular sciences · 2025Review
- miRNA-targeted auxin nuclear signalling elements orchestrate flower fate and drought response in yellow lupine.Scientific reports · 2025Article
- CRISPR/Cas genome editing in soybean: challenges and new insights to overcome existing bottlenecks.Journal of advanced research · 2025Review
- Roles of microRNAs and histone modifications in enhancing stress tolerance in soybean and their applications in molecular breeding.Breeding science · 2025Article
- Genome-wide identification of the Sec14 gene family and the response to salt and drought stress in soybean (Glycine max).BMC genomics · 2025Article
- Validation of suitable reference microRNAs for qRT-PCR inFrontiers in plant science · 2025Article
- Functional Identification of miR2119 TargetingJournal of agricultural and food chemistry · 2024Article
- Review
- The miRNA-mRNA Regulatory Modules ofInternational journal of molecular sciences · 2023Article
- MicroRNA: A Dynamic Player from Signalling to Abiotic Tolerance in Plants.International journal of molecular sciences · 2023Review
- Developing drought-smart, ready-to-grow future crops.The plant genome · 2023Review
- Gene Editing for Plant Resistance to Abiotic Factors: A Systematic Review.Plants (Basel, Switzerland) · 2023Review
- Advances in CRISPR/Cas9-based research related to soybean [Frontiers in plant science · 2023Review
- miR2119, a Novel Transcriptional Regulator, Plays a Positive Role in Woody Plant Drought Tolerance by Mediating the Degradation of theInternational journal of molecular sciences · 2022Article
- Progress in Soybean Genetic Transformation Over the Last Decade.Frontiers in plant science · 2022Review
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Authors and funding
13 authors at 1 institution in 1 country.
Funding
Abstract
backgroundDrought conditions adversely affect soybean growth, resulting in severe yield losses worldwide. Increasing experimental evidence indicates miRNAs are important post-transcriptional regulators of gene expression. However, the drought-responsive molecular mechanism underlying miRNA-mRNA interactions remains largely uncharacterized in soybean. Meanwhile, the miRNA-regulated drought response pathways based on multi-omics approaches remain elusive.
resultsWe combined sRNA, transcriptome and degradome sequencing to elucidate the complex regulatory mechanism mediating soybean drought resistance. One-thousand transcripts from 384 target genes of 365 miRNAs, which were enriched in the peroxisome, were validated by degradome-seq. An integrated analysis showed 42 miRNA-target pairs exhibited inversely related expression profiles. Among these pairs, a strong induction of gma-miR398c as a major gene negatively regulates multiple peroxisome-related genes (GmCSD1a/b, GmCSD2a/b/c and GmCCS). Meanwhile, we detected that alternative splicing of GmCSD1a/b might affect soybean drought tolerance by bypassing gma-miR398c regulation. Overexpressing gma-miR398c in Arabidopsis thaliana L. resulted in decreased percentage germination, increased leaf water loss, and reduced survival under water deficiency, which displayed sensitivity to drought during seed germination and seedling growth. Furthermore, overexpressing gma-miR398c in soybean decreased GmCSD1a/b, GmCSD2a/b/c and GmCCS expression, which weakened the ability to scavenge O
conclusionThe study indicates that gma-miR398c negatively regulates soybean drought tolerance, and provides novel insights useful for breeding programs to improve drought resistance by CRISPR technology.
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